Set Up The Targets - Applied Biosystems 7500 Getting Started

Chapter 2 Design the Standard Curve Experiment

Set Up the Targets

For More
Information

Set Up the Targets

About the
Example
Experiment
Complete the
Targets Screen
Notes
24
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
For more information on:
• Completing the Methods & Materials screen – Open the 7500 Software Help by
clicking or pressing F1.
• Using Advanced Setup – See
• Using other quantitation methods – Refer to the Applied Biosystems 7500/7500 Fast
Real-Time PCR Systems Getting Started Guide for Relative Standard Curve and
Comparative C Experiments.
T
• TaqMan and SYBR Green reagents – Refer to the Applied Biosystems Real-Time
PCR Systems Reagent Guide.
• PCR, including singleplex vs. multiplex PCR and 1-step vs. 2-step RT PCR – Refer
to the Applied Biosystems Real-Time PCR Systems Reagent Guide.
In the Targets screen, you enter the number of targets you want to quantify in the PCR
reaction plate, then set up the experiments for each target.
In the standard curve example experiment:
• One target is quantified in the reaction plate.
• The Set Up Standards check box is selected. When this check box is selected, the
software automatically displays the Standards screen after you complete the Targets
screen. In the Standards screen, you can set up a standard curve for the target
experiment (see "Set Up the Standards" on page
• The Target 1 experiment is set up for the target you are studying. For the example
experiment, the target is the RNase P gene.
1. Click the How many targets do you want to quantify in the reaction plate? field,
then enter 1.
The number of rows in the targets table is updated with the number you
Note:
entered.
2. Select the Set Up Standards check box to set up standards for the target
experiment.
The Set Up Standards check box is selected by default.
Note:
3. Set up the Target 1 experiment:
a. Click the Enter Target Name cell, then enter RNase P TAMRA.
In the Reporter drop-down list, select FAM (default).
b.
In the Quencher drop-down list, select TAMRA.
c.
d. In the Color field, leave the default.
"Advanced Setup Workflow" on page
26).
98.
Experiments