Example Experiment Workflow - Applied Biosystems 7500 Getting Started

Chapter 1 Get Started

Example Experiment Workflow

Example Experiment Workflow

Notes
14
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Example Files in the Experiments Folder
The experiments folder of the 7500 software contains several example files that you can
reference when analyzing your own data. The following example files install with the
7500 software:
• Comparative Ct Example.eds
• Comparative Ct Study Example.edm
• Comparative Ct Study (Biological Groups).edm
• Genotyping Example.eds
• Presence Absence Example.eds
• Relative Standard Curve Example.eds
• Standard Curve Example.eds
Be sure to use the Standard Curve Example.eds file when you perform the
IMPORTANT!
procedures in this guide.
1. Create a new experiment.
2. Define the experiment properties.
3. Define the methods and materials.
4. Set up the targets.
5. Set up the standards.
6. Set up the samples.
7. Set up the run method.
8. Review the reaction setup.
9. Order materials for the experiment.
10.Finish the Design Wizard.
1. Prepare the sample dilutions.
2. Prepare the standard dilution series.
3. Prepare the reaction mix for each target assay.
4. Prepare the reaction plate.
Start Experiment
Design the Experiment (Chapter 2)
Prepare the Reactions (Chapter 3)
(see page 15)
Experiments