Design The Standard Curve Experiment - Applied Biosystems 7500 Getting Started

This chapter covers:
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Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
Design the Standard Curve
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Chapter Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
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Create a New Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
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Define the Experiment Properties . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
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Define the Methods and Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
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Set Up the Targets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
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Set Up the Standards. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
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Set Up the Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
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Set Up the Run Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
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Review the Reaction Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
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Order Materials for the Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
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Finish the Design Wizard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
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Chapter 2
Experiment
17