Designing An Rq Experiment - Applied Biosystems 7500 Getting Started Manual

Introduction
and Example
RQ Experiment
Designing
an RQ
Experiment
Performing
Primer Extended on mRNA
5´ 3´ Reverse
5´ cDNA
Reverse
Primer
Oligo d(T) or random hexamer
Synthesis of 1st cDNA strand
3´ 5´ cDNA
Transcription
Generating
Data from
STANDARD TANDAR
RQ Plates
Standard
Performing
an RQ Study
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide

Designing an RQ Experiment

Generating
Data from
OR
RQ Plates
Fast
Select the PCR method
Specify the components of an
RQ experiment
Select the chemistry
Select one-step or
two-step RT-PCR
Choose the probes
and primers
Chapter 2
2
See page 8
See page 9
See page 11
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