About Standard Curve Experiments - Applied Biosystems 7500 Getting Started

About Standard Curve Experiments

Real-Time PCR
Standard curve experiments are real-time PCR experiments. In real-time PCR
Experiments
experiments:
Note:
using the 7500/7500 Fast system, including setup, run, and analysis.
About Standard The standard curve method is used to determine the absolute target quantity in samples.
Curve With the standard curve method, the 7500 software measures amplification of the target
Experiments in samples and in a standard dilution series. Data from the standard dilution series are
used to generate the standard curve. Using the standard curve, the software interpolates
the absolute quantity of target in the samples.
Components
The following components are required when setting up PCR reactions for standard
curve experiments:
Notes
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
• The instrument monitors the progress of the PCR as it occurs.
• Data are collected throughout the PCR process.
• Reactions are characterized by the point in time during cycling when amplification
of a target is first detected.
In this guide, the term experiment refers to the entire process of performing a run
• Sample – The sample in which the quantity of the target is unknown.
• Standard – A sample that contains known standard quantities; used in quantitation
experiments to generate standard curves.
• Standard dilution series – A set of standards containing a range of known
quantities. The standard dilution series is prepared by serially diluting standards.
• Replicates – The total number of identical reactions containing identical samples,
components, and volumes.
• Negative Controls – Wells that contain water or buffer instead of sample template.
No amplification of the target should occur in negative control wells.
Chapter 1 Get Started

About Standard Curve Experiments

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