Analyzing Data In An Rq Study - Applied Biosystems 7500 Getting Started Manual

Introduction
and Example
RQ Experiment
Designing
an RQ
Experiment
Performing
Primer Extended on mRNA
5´ 3´ Reverse
5´ cDNA
Reverse
Primer
Oligo d(T) or random hexamer
Synthesis of 1st cDNA strand
3´ 5´ cDNA
Transcription
Generating
Data from
STANDARD TANDAR
RQ Plates
Standard
Performing
an RQ Study
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide

Analyzing Data in an RQ Study

Generating
Data from
OR
RQ Plates
Fast
Chapter 6
Create a new
See page 60
RQ Study document
Configure
See page 63
analysis settings
Adjust the
See page 65
baseline and
threshold
Analyze and
See page 70
view results
If necessary,
See page 75
omit samples
Export AQ
See page 78
Plate document,
if desired
6
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