Viewing Plus/Minus Results - Applied Biosystems 7300 Getting Started Manual

Chapter 6 Performing the Plus/Minus Post-Read Run

Viewing Plus/Minus Results

Sample Experiment
The image below displays the amplification plot for well A1, the No Amplification Control (NAC), which contains blocked
IPC and no target template. This plot demonstrates that there is no amplification for IPC or the E. coli target sequence.
Notes
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Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Plus/Minus Assay Getting Started Guide
The following Example Experiment boxes show the amplification plots of each type of
result (NAC, NTC, plus (+), minus (− ), and undetermined (?)).
The amplification plots are obtained from the AQ plate document used to amplify
Note:
the samples in the 96-well plate.
Well A1
No E. coli present
Blocked IPC, no amplification