Combinations Of Optical Parts - Nikon ECLIPSE Si Instructions Manual

3.11

Combinations of Optical Parts

Compatibility between objectives and microscopy methods
Objective
E Plan 4x
E Plan 10x
E Plan 40x
E Plan 60x
E Plan 100x Oil
Achromat DL 10x Ph1
Achromat DL 20x LWD Ph1
Achromat DL 40x Ph2
Achromat DL 100x Oil Ph3
20x objectives for bright-field microscopy
Plan 20x objectives can be used for bright-field microscopy with 20x magnification.
Combinations of objectives and 10x (field number 20) eyepieces
Objective
E Plan 4x
E Plan 10x
E Plan 40x
E Plan 60x
E Plan 100x Oil
Achromat DL 10x Ph1
Achromat DL 20x LWD Ph1
Achromat DL 40x Ph2
Achromat DL 100x Oil Ph3
Chapter 3 Detailed Explanation
Compatibility between optical parts and microscopy methods
Magnification Bright-field
4x
10x
40x
60x
100x
10x
20x
40x
100x
Compatibility between optical parts and microscopy methods
Total
Magnification Magnification
4x
10x
40x
60x
100x 1000
10x
20x
40x
100x 1000
Phase
Contrast Polarizing
S -
S -
S -
S -
S -
S R
S R
S R
S R
(R: Recommended; S: Suitable; U: Usable; -: Incompatible)
Real
Numerical
Field
Aperture
(x) (mm)
40 0.1
100 0.25
400 0.65
600 0.8
1.25
100 0.25
200 0.40
400 0.65
1.25
96
Simple Diascopic
Fluorescence
U S
U S
U S
U -
U -
U U
U U
U U
U -
Depth of Resolving
Focus Power
(μm) (μm)
5 115 2.8
2 18.4 1.1
0.5 2 0.4
0.3 1.2 0.3
0.2 0.7 0.2
2 18.4 1.1
1 6.1 0.7
0.5 2 0.4
0.2 0.7 0.2
Dark-field
-
U
S
-
-
U
S
S
-
Working
Distance
(mm)
30.00
7.00
0.65
0.3
0.23
7.00
3.9
0.65
0.23