When To Perform Dvd Function - Thermo Scientific VF-F20-A Operating Manual

6.7.2

When to Perform DVD Function

Integral Fraction Collector FT (VF-F20-A) Operating Manual
No. Description
1 Delay capillary
2 Fraction collection valve
When collection is triggered during a run, the fraction collection valve is
switched from waste position to collection position using the following
delay time calculations:
•
Peak-based:
Start of fraction collection: t = t
End of fraction collection: t = t
•
Time-based:
Start of fraction collection: t = t
End of fraction collection: t = t
It is necessary to re-measure the delay volume if any of the below
conditions are met.
•
Initial installation of a new HPLC system with an Integral Fraction
Collector (VF-F20-A)
•
Replacing a detector flow cell
•
Replacing a delay capillary
•
Replacing a fraction collection valve
•
Replacing an air bubble sensor
Important!
•
Only qualified Thermo Fisher engineer can replace the fraction
collection valve and the air bubble sensor.
•
Never move or uninstall the fitting on port 3 of fraction collection
valve. The flow path on this port have been performed calibration
during in-house production process or in-field service.
No. Description
3 Air bubble sensor
+ Delay time
peak start
+ Delay time
peak end
+ Delay time
collection time window start
+ Delay time
collection time window end
•
6 Operation
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