Thermo Scientific VF-F20-A Operating Manual page 114

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6 Operation
Page 114
Parameters
Purge flush pump
Needle wash and rinse
External needle
wash
Purge needle
wash
Internal needle
rinse
Integral Fraction Collector FT (VF-F20-A) Operating Manual
Description
By selecting the Purge Flush Pump button on
the ePanel FC tab, all flow path from flush
solvent reservoir to flush pump and flush
buffer loop is filled up with flush solvent.
For details on washing the needle with fresh
needle wash liquid, see Performing A Needle
Wash Cycle ( page 162).
Select External Needle Wash button on
ePanel FC tab to move the needle to wash
port and perform a manual needle wash
procedure with the wash time and wash
speed that is currently defined by External
Needle Wash Speed and External Needle
Wash Time in command list (F8).
To purge the needle wash system with fresh
needle wash liquid, for example after
replacing the needle wash liquid, you can
select the Purge Needle Wash button on the
ePanel FC tab for the device.
Chromeleon turns on the needle wash pump
and performs a needle wash cycle with
dedicated wash settings for purging the
needle wash system.
After purging, the needle wash pump is
turned off again and the previous wash
settings are restored.
As a standard, the needle wash pump is
turned off (Wash Pump = Off ).
Perform a manual internal needle rinse.
Select the Internal Needle Rinse button to
perform a manual needle rinse cycle using
mobile phase. The needle will be inserted into
needle wash port during rinsing and 2x the
needle volume (30 µL) will be rinsed with flow
from the HPLC pump. The duration of rinse
with therefore depend on the set flow rate of
the HPLC pump.
If the pump motor is off or the flow rate is set
to 0 mL/min and Internal Needle Rinse is
triggered an error message will be issued
(Code 9055. Internal needle rinse failed.
Pump speed is 0.).