Thermo Scientific VF-F20-A Operating Manual page 7

5.9
5.10 Turning On the Device .......................................................................................... 93
5.11 Setting Up the Device in the Software .................................................................. 94
6 Operation ........................................................................................96
6.1
6.2
6.3
6.4
6.5
6.6
6.7
6.8
6.9
6.10 Shutting Down the Device .................................................................................. 138
Integral Fraction Collector FT (VF-F20-A) Operating Manual
Flush System ......................................................................................................... 86
5.9.1 Choosing the Flush Solvent ...................................................................... 86
5.9.2 Connecting Flush Solvent Reservoir ......................................................... 86
5.9.3 Connecting the Delay Capillary ................................................................ 90
5.9.4 Connecting the Flush Buffer Loop ............................................................ 91
Introduction to this Chapter ................................................................................. 97
Safety Guidelines for Operation ........................................................................... 98
Control Elements ................................................................................................ 100
6.3.1 Keypad.................................................................................................... 100
6.3.2 Status Indicators .................................................................................... 102
Power On/Off Control ......................................................................................... 104
Preparing the Device for Operation .................................................................... 105
6.5.1 Thermostatting the Fraction Collector Compartment ........................... 107
6.5.2 Loading the Carousel.............................................................................. 107
6.5.3 Rack Type Settings ................................................................................. 111
6.5.4 Needle Height Setting ............................................................................ 111
Important Operating Parameters ....................................................................... 113
Delay Volume Determination (DVD) ................................................................... 120
6.7.1 Delay Volume and Time ......................................................................... 120
6.7.2 When to Perform DVD Function ............................................................ 121
6.7.3 How to Perform DVD Function .............................................................. 122
6.7.4 Delay Volume Capillary Drop Down Menu ............................................ 124
6.7.5 Vanquish Modules enabling Delay Volume Determination ................... 125
6.7.6 Vanquish Detector Flow Cells enabling Delay Volume Determination.. 126
6.7.7 Delay Volume Calculator ........................................................................ 127
Vial Pusher Positions ........................................................................................... 132
6.8.1 Adjust to AboveVial Pusher Position...................................................... 133
6.8.2 Adjust to InVial Pusher Position ............................................................. 135
Optimizing the Performance of the Device ........................................................ 137
6.10.1 Short-Term Shutdown (Interruption of operation) ............................... 138
6.10.2 Long-Term Shutdown ............................................................................. 139
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