Summary of Contents for Thermo Scientific 7470 Series
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Model 7450 and 7470 Series CryoMed Controlled Rate Freezer Operating and Maintenance Manual 7027452 Rev. 4 Visit us online to register your warranty www.thermoscientific.com/labwarranty...
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28816/FR-2371 8/29/12 Added ferrite - parts list, schematics, wiring diagrams 28576/FR-2357 7/31/12 Original - Release 4 with new printer power supply 28511/FR-2371 7/31/12 Original - Release 4 with new printer power supply ECR/ECN DATE DESCRIPTION Thermo Scientific Controlled Rate Freezer...
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Always use the proper protective equipment (clothing, gloves, goggles, etc.) 4 Always dissipate extreme cold or heat and wear protective clothing. 4 Always follow good hygiene practices. 4 Each individual is responsible for his or her own safety. Thermo Scientific Controlled Rate Freezer...
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We can also provide you with a quotation on our Extended Warranty for your Thermo Scientific products. Whatever Thermo Scientific products you need or use, we will be happy to discuss your applications. If you are experiencing technical problems, working together, we will help you locate the problem and, chances are, correct it yourself...over the telephone without a service...
Displays There are three displays on the Controlled Rate Freezer control panel: Chamber and Sample temperature, ranging from +54° to -199°C, the LCD Display for profile status, messages, and freezing profile editing and viewing. Controlled Rate Freezer Thermo Scientific...
LED flashes in addition to an audible tone. See Alarms, Section 2. Output Graphs and cycle data, as well as additional information, are printed out at the top of the unit on a thermal printer in real-time mode. Thermo Scientific Controlled Rate Freezer...
When closing the freezer door, be sure to turn the handle 180° in rotation to fully seal the door. On/Off Switch (mains disconnect) RS232 Interface Power Inlet Tank Hose Connection Figure 2-1. Connections on Unit Back Thermo Scientific Controlled Rate Freezer...
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Section 2 Set-Up and Operation Figure 2-2. Clearance for All Units Controlled Rate Freezer Thermo Scientific...
2 Pollution Degree describes the amount of conductive pollution present in the operating environment. Pollution Degree 2 assumes that normally only non-conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation. Thermo Scientific Controlled Rate Freezer...
4. To choose a profile, scroll through the selections using the up/down arrow keys. The profiles are displayed in a loop. Pressing either of the arrow keys consecutively brings the list back to the starting point. Controlled Rate Freezer Thermo Scientific...
5. Press the Run key. If the chosen profile begins with a Wait, the Run key must be pressed again to continue, after the appropriate temperature is reached. 6. The mode indicator is lit, showing the status of the profile. Thermo Scientific Controlled Rate Freezer...
However, Access Code protection is not required on an End step. If a power outage interrupts a run, the profile will restart from the point of interruption when power is restored. See Alarms in this section. Controlled Rate Freezer Thermo Scientific...
1. Locate the arrow to the left of the option that is on the top line of the display. Move the profile (USER 1 through USER 10) that you wish to view or edit to the top line by pressing the up or down arrow keys. Thermo Scientific Controlled Rate Freezer...
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Step 1 contains a command with a cursor blinking just to the right of a left pointing arrow (← __ ). With the cursor in this position, use the up or down arrow keys to scroll through the available commands. Controlled Rate Freezer Thermo Scientific...
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For example, if the chamber set point is 30°C, the controller will ramp to 31°C until the setpoint is reached. Graph print-outs show this ramp Figure 2-4. Ramp Settings setting. Thermo Scientific Controlled Rate Freezer...
When the desired User Profile is showing on the first line of the display, press the Enter key to complete the paste operation, or press Back to cancel. 2-10 Controlled Rate Freezer Thermo Scientific...
Run start time and date Profile name 1234 cell culture media User information D.M.S.O. Time/Temp scale Chamber temp Sample temp Profile steps listed as they occur Temp tracking alarm Profile line Figure 2-4. Print-outs Thermo Scientific Controlled Rate Freezer 2-11...
Chamber thermocouple is corrected. Once resolved, the second depression of the Silence key will turn off the alarm indicator, clear the alarm message from the fourth line of the LCD display, and enable all operating functions. 2-12 Controlled Rate Freezer Thermo Scientific...
Sample thermocouple is corrected. Once resolved, the second depression of the Silence key will turn off the alarm indicator, clear the alarm message from the fourth line of the LCD display, and enable the Run key. Thermo Scientific Controlled Rate Freezer 2-13...
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Once resolved, the second depression of the Silence key will turn off the alarm indicator, clear the alarm message from the fourth line of the LCD display, and enable the Run key. 2-14 Controlled Rate Freezer Thermo Scientific...
However, the alarm message remains on the fourth display line until the Silence key is depressed. If the temperature of either probe continuously exceeds the pre-set limit temperatures for more than 10 minutes, the unit will immediately switch to Idle Mode and disable all functions. Thermo Scientific Controlled Rate Freezer 2-15...
Alarm LED will stop flashing and the audible alarm will silence (if the Silence key has not already been pressed). However, the alarm message will remain on the fourth display line until the Silence key is pressed a second time. 2-16 Controlled Rate Freezer Thermo Scientific...
The controller will maintain the temperature of the final step in the profile run until the run is stopped by the operator pressing the Back key. Caution If performing successive runs, dry the gaskets and heat breaks between each run. Thermo Scientific Controlled Rate Freezer 2-17...
Note: The printer continues to print current Run data even though the ‘Printer Buffer Full’ alarm has occurred. IVF Sample Holders Included with the IVF Controlled Rate Freezer are a straw holder, vial holder, cane holder and access port plugs. 2-18 Controlled Rate Freezer Thermo Scientific...
4. To install the straw probe, insert the probe down the center of the button indicated below. Slide the probe down through the holder until it feeds through the center straw. Thermo Scientific Controlled Rate Freezer 2-19...
If the cane holder is used with a sample probe, use the standard 1.2ml vial probe. Connect the probe to the internal sample probe connection. Caution Do not connect two sample probes at the same time. 2-20 Controlled Rate Freezer Thermo Scientific...
3. Tighten the small hex socket set screws when the adjustment is complete, just until the screws lightly touch the rods to avoid damaging the rod threads. Figure 2-7. Vial Holder Adjustment Figure 2-8. Straw Holder Thermo Scientific Controlled Rate Freezer 2-21...
Enter and Back keys, and changing the digit using the up/down arrow keys. The default Access Code (security disabled) is 0 0 0 0. Each digit may be set over a range of 0 to 9. Exit this menu by using the Back key. Thermo Scientific Controlled Rate Freezer...
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A duplicate of the print-out made during the Run. The print-out reflects the actual time and date of the run, rather than the current time and date. Note The unit will store at least 2 hours of data. Controlled Rate Freezer Thermo Scientific...
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0.5000 to 1.9995 in 0.0005 increments. Adjust the probe scaler for a -196°C temperature display reading with the probe in liquid nitrogen. Warning Contact of liquid nitrogen with the skin or eyes may cause serious freezing (frostbite) injury. Thermo Scientific Controlled Rate Freezer...
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‘Offset’ and the ‘Scaler’ adjustments. 3. Service This menu is for use by qualified service personnel. Entry into this menu has a required service access code. Warning This area may be accessed by qualified service personnel only. Controlled Rate Freezer Thermo Scientific...
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This menu item enables a thermal simulator that allows the unit to run freezing profiles in a realistic manner without actually using the chamber heater or cooling solenoids. The simulator uses separate parameters for heating and cooling effects that approximate the performance of the real chamber. Thermo Scientific Controlled Rate Freezer...
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([L] 1.7 cu ft, [M] 1.2 cu ft, and [S] 0.6 cu ft). The following is a listing of parameters with their defaults. • Size: L, M, S (default is M) • Cycle Time adjusted over a range of 500 to 2000mS (default is 1000mS) Controlled Rate Freezer Thermo Scientific...
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Simulation (Off; Cool: 1S, 10S, 90C/m; Heat: 1S, 45S, 9C/m) • Thermal Controller (Size M, 1000mS, P: 0.25, I: 0.075, D: 0.2, PR: 0.01, CP: 4, CM: 1.0, C) • Tracking Alarms (5C, 1m) Note User Profiles are not affected. Thermo Scientific Controlled Rate Freezer...
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(Time - 1 minute increments) and temperature difference from setpoint (Delta - 1°C increments). The Time parameter can be adjusted over a range from a 1 to 99 minute range. Exit the menu using the Back key. Controlled Rate Freezer Thermo Scientific...
After the cabinet has been rinsed, spray with 70% alcohol. 4. Allow the cabinet door to remain open for the alcohol to evaporate. 5. Plug the freezer in and turn the power switch on. Thermo Scientific Controlled Rate Freezer...
9. Connect the Sample probe to the connector, if applicable. 10. All chamber parts must be re-installed for consistent uniformity and airflow. Connect the unit to the power source and turn it on. 11. The freezer is ready to be returned to service. Controlled Rate Freezer Thermo Scientific...
Clean Gasket/Heat Dry any moisture from the door gasket and heat breaks between each run, using a soft cloth, to prevent ice build-up which could reduce the Break effectiveness of the door seal. Thermo Scientific Controlled Rate Freezer...
4 inches (10cm) through the paper cutter. 5. Press the paper feed switch until the paper Insertion feeds in straight and smoothly. Slot The printing surface is the outside of the thermal paper roll. Thermo Scientific Controlled Rate Freezer...
Rack and Holder Vial Placement for each size chamber 0.6 cu. ft. 1.2 cu. ft. 1.7 cu. ft. Models Models Models 7450/7451 7452/7453 7454/7455 7456/7457 7458/7459 7474 7472, 7475 7473, 7476 Thermo Scientific Controlled Rate Freezer...
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Maximum of 12.0°C / min (same conditions as above) Average of 9.0°C / min (same conditions as above, range -40°C to -12°C) Note Rates vary based on load, LN pressure, unit size, and voltage. Controlled Rate Freezer Thermo Scientific...
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Heater overtemperature protection: At a temperature level of 93°C, a manual reset device interrupts power to the heating element. External Connections Power entry PC interface port LN2 connection 1/2” flare External thermocouple jack (IVF models only) Sample access (IVF models only) Thermo Scientific Controlled Rate Freezer...
CRF units through the same PC, it is recommended to minimize the in- active windows or instances of open software not being used. This reduces the load on the CPU and improves the efficiency/productivity of the software. Thermo Scientific Controlled Rate Freezer 10-1...
3. When the user inserts the CD into the CD/DVD drive, Figure 10-2 will appear. Select ‘Open Folder to view files’. Select PC Interface.exe. Your view may vary slightly. Figure 10-2. Window on CD Insertion 10-2 Controlled Rate Freezer Thermo Scientific...
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10-4). If not, you will see an error message. Select Next. 7. Check the little window if a desktop icon is required. See Figure 10-5. Select Next. Figure 10-4. Installation Location Figure 10-5. Desktop Icon Thermo Scientific Controlled Rate Freezer 10-3...
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10. If Java Runtime Environment is either not installed or is an older version, then the window shown in Figure 10-8 will display. Select Yes to install a new or newer version of Java, or No to cancel for lack of admin access rights. 10-4 Controlled Rate Freezer Thermo Scientific...
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12. Allow the Java installation process to complete. It may take a few minutes. The next window will open automatically. Figure 10-10. Java Installation Progress Figure 10-11. Installation Completion 13. After installation is complete (Figure 10-11), select Close to finish. Thermo Scientific Controlled Rate Freezer 10-5...
15. If Java is not installed or the version is too old, the error below will display and the PC software will not open. Figure 10-13. Java Not Installed Error Login The icon at right appears in the default directory after the application is successfully installed. 10-6 Controlled Rate Freezer Thermo Scientific...
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(‘@’ ‘$’ and ‘_’ only) can be added after the first character. • Password must be alphanumeric from third character onwards. • Username cannot have spaces or linefeed. • "Please enter Username” message displays if Username field is blank. Thermo Scientific Controlled Rate Freezer 10-7...
This message appears when the password field is blank. This message appears when the user enters incorrect username and password. Setup The Home Page screen opens as shown below: Figure 10-2. Home Page of PC Software 10-8 Controlled Rate Freezer Thermo Scientific...
The user can perform the following functions as described. Figure 10-3. Function Selections Run Operation Run: The Run command opens communication with the freezer through the COM port. Profile commands are downloaded and the program executed. Figure 10-4. Run Screen Thermo Scientific Controlled Rate Freezer 10-9...
The user must enter a Run file name that begins with a letter. Special characters will not be allowed. The length of the name cannot exceed 30 characters. Figure 10-5. Run File Name 10-10 Controlled Rate Freezer Thermo Scientific...
Profile Name*- Indicates the profile selected (.prg file) by the user for the run. • Sample type - Enter the type of sample being frozen. The description cannot exceed 30 characters. Note Profile Name, Date/Time, and Operator fields are not editable by user. Thermo Scientific Controlled Rate Freezer 10-11...
WAIT step. • Calculates the time only for profiles having ramp and hold steps. • Does not calculate during manual override, move to, loop and jump functions. • Displays time in minutes and seconds. 10-12 Controlled Rate Freezer Thermo Scientific...
Y-axis displays temperature in degrees Celsius (°C). Default setting for Y-axis is 5°C. • Allows the user to zoom in and out. Graph is adjusted based on the temperature selected. Press Ctrl + Mouse Left/Right button to pan around graph. Thermo Scientific Controlled Rate Freezer 10-13...
COM ports, to which the CRF has to be connected. The ports which are not available at that time will be inactive (hidden). The COM port Selection window is shown at right. Figure 10-14. Select Port 10-14 Controlled Rate Freezer Thermo Scientific...
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When the user clicks “Click here to continue” the application will send a run (22 decimal) command to the freezer that causes the freezer to move to the next step in the profile. Figure 10-18. Continue Thermo Scientific Controlled Rate Freezer 10-15...
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Remote profile in ‘Stand-alone’ mode. Clicking the Stop button at this time stops only the data capture. Choose ‘Continue cap- turing data’ or ‘Stop capturing data’. No other commands can be sent from the PC. 10-16 Controlled Rate Freezer Thermo Scientific...
The manual override displays three modes of temperature variation: Cool: The override Cool button energizes one solenoid. Cool+: The override Cool+ button energizes both solenoids. Warm: The override Warm button turns the heater ON. Thermo Scientific Controlled Rate Freezer 10-17...
Figure 10-28. Completed window bar. This will terminate the profile. 7. To warm the chamber to room temperature, select and run preset profile #7 (preset_warming). This profile will return the chamber to a temperature of 25°C. 10-18 Controlled Rate Freezer Thermo Scientific...
Alarms occurrence details with alarm type and time of occurrence • Page numbers at bottom left corner of print document • "Thermo Scientific" logo at bottom right corner of print document. Profile Graph contains: • "Data file" profile name with directory details •...
(5°C). • "Temperature is Low: Click OK to Silence” message appears when chamber temperature is less than programmed temperature by a pre- determined amount (5°C). 10-20 Controlled Rate Freezer Thermo Scientific...
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10 seconds. The graph on the Run screen stops plotting when there is a power failure and user will be prompted if failure duration is more than 10 seconds. Thermo Scientific Controlled Rate Freezer 10-21...
Two Run screens can be restored for freezers that were running during the PC power failure. As the freezer did not experience the power failure, no alarm will be recorded. 10-22 Controlled Rate Freezer Thermo Scientific...
3 seconds. All freezer status and temperature displays operate normally. The message 'Running Remote Profile' is displayed at the freezer, as well as the current step information alternated with any alarm messages. Thermo Scientific Controlled Rate Freezer 10-23...
Hold Command: Allows the user to enter a temperature range between 50°C to -180°C. The edit screen is shown below. Press Ctrl + Mouse Left/Right button to pan around graph. Figure 10-31. Edit Screen 10-24 Controlled Rate Freezer Thermo Scientific...
Wait For temperature Figure 10-33. Wait For Screen WAIT AT CHAMBER=X.X°C UNTIL SAMPLE=Y.Y°C. X.X is Chamber set temperature and Y.Y is the Sample trip temperature. The default Sample trip temperature is 1°C above Chamber temperature. Thermo Scientific Controlled Rate Freezer 10-25...
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The time can be set over a range of 1 to 99 minutes, and the temperature can be set over a range of -180°C to +50°C with a setting resolution of 0.1°C. Note Chamber and Sample selection are deactivated here. 10-26 Controlled Rate Freezer Thermo Scientific...
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3. The defined commands and temperatures are displayed in Profile Steps. 4. Click Delete if the user wants to delete a profile step. Note The user can add notes in the Notes section, if desired. Thermo Scientific Controlled Rate Freezer 10-27...
3. If the user selects WAIT command, the CRF (chamber) waits at 0°C or at user defined temperature. If the user selects WAIT FOR command, the chamber temperature waits at 0°C until sample temperature is 1°C. 10-28 Controlled Rate Freezer Thermo Scientific...
1. Click Open Profile. A dialog box with title “CRF PC Companion: Open” is displayed which allows only .prg file to be selected. The steps appear in the program listing window and the graph is also displayed. Thermo Scientific Controlled Rate Freezer 10-29...
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6. Delete button is used to delete a step. 7. Delete All button is used to delete all steps at a time. When the user clicks the Delete All button, a confirmation window is displayed as shown below. 10-30 Controlled Rate Freezer Thermo Scientific...
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Notes will be printed with the Run data. 13. Make the required changes and Click Save. A Save window opens. Figure 10-40. Save 14. Click Save. The modified changes of the profile step are saved. Thermo Scientific Controlled Rate Freezer 10-31...
To view a specific location on the graph, point at cursor, press and hold the left mouse button, and drag the cursor to the desired graph position. 1. Axis: i. X-Axis will be displayed as Minutes ii. Y- Axis will be displayed as temperature (°C) 10-32 Controlled Rate Freezer Thermo Scientific...
+0.0°C/min to +99.9°C/m (Ramp). This message appears when the sample temperature is not greater than the “Invalid Sample temperature value entered. Value must be chamber temperature (Wait for). greater than the Chamber temperature” (continued) Thermo Scientific Controlled Rate Freezer 10-33...
Review enables the user to perform the following functions: • Review a run profile • Compare two run profiles • Export the file in a .CSV format. • Print a profile Figure 10-43. Review Screen 10-34 Controlled Rate Freezer Thermo Scientific...
Compare: This screen allows the user to compare two run profiles. The profiles are named as Run File A and Run File B. The user can compare information fields, profile steps, analyse the graph plots of two profiles and the chamber and sample temperatures respectively. Thermo Scientific Controlled Rate Freezer 10-35...
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Section 10 PC Interface Software User Controls (continued) Figure 10-44. Compare Profiles Figure 10-45. Export • Export: The export icon allows the user to export the file into the local system in the .CSV format. 10-36 Controlled Rate Freezer Thermo Scientific...
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Initially will be blank and not editable b. Populates the profile name once the Run file is loaded 2. Date/ Time: a. Initially will be blank and not editable b. Populates the Date/Time once the Run file is loaded Thermo Scientific Controlled Rate Freezer 10-37...
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Initially will be blank b. Populates Profile steps once the Run file is loaded 9. Notes: a. Initially will be blank b. Populates notes once the Run file is loaded if data is available in run file. 10-38 Controlled Rate Freezer Thermo Scientific...
Plot Colors The Plot Colors screen allows a user to set the colors of the graph. Figure 10-47. Plot Colors The Plot Colors window indicates the graph color choices. Figure 10-48. Plot Color Choices Thermo Scientific Controlled Rate Freezer 10-39...
This screen enables the user to change the default directory settings. The user can define the default profile directory and default run directory to store the program profiles and run profiles. Figure 10-49. Default Directories 10-40 Controlled Rate Freezer Thermo Scientific...
Users This section enables a user to add new users, modify passwords and delete existing users. The following image shows the “add new user” screen. Figure 10-49. Add New User Thermo Scientific Controlled Rate Freezer 10-41...
To access the application in user’s mode, a new user will have to be set up. Users cannot be imported from other applications or previous versions of Thermo Scientific CRF software. • All existing users will be displayed in a list/scrollbar.
Sample Type. The information fields that are added are available in subsequent runs. The user can add 5 additional fields. The total number of information fields that can be defined is 12. Figure 10-52. Add Information Fields Thermo Scientific Controlled Rate Freezer 10-43...
PC software (unit connected or unit not connected), the port connected and the username in the bottom right corner of the window. Figure 10-53 shows the status bar. Figure 10-53. Status Bar 10-44 Controlled Rate Freezer Thermo Scientific...
The Help section assists the user in navigating through the PC Software. Figure 10-54. Information About This section provides information for Thermo Fisher, the OS compatibility, and the software version of the application. Figure 10-55. Help File Thermo Scientific Controlled Rate Freezer 10-45...
Note Because argon is an inert gas whose physical properties are very similar to those of nitrogen, the precautions and safe practices for handling and use of liquid argon are the same as those for liquid nitrogen. s Thermo Scientific Controlled Rate Freezer AA-1...
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Rapid warming of the affected part is best achieved by using water at 108°F. Under no circumstance should the water be over 112°F, nor should the frozen part be rubbed either before or after rewarming. The patient should neither smoke nor drink alcohol. AA-2 Controlled Rate Freezer Thermo Scientific...