Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual page 99

Perform ROI/uniformity, background, dye, and normalization calibrations for the TaqMan
This procedure explains how to create all of the array cards required to calibrate the block, but not all of
them are required for at each calibration. Before preparing array cards for calibration, determine which
calibrations are required (see "Calibration and verification schedule"
Note: For more information on the array card loading procedure, contact Technical Support.
1. Remove the tubes of calibration solutions from the freezer, allow them to thaw, then vortex the
tubes to mix the contents well.
2. Remove the TaqMan
3. Mark the side of the empty array cards with the following items:
• Background
• FAM ™
• ROI
• ROX ™
Location to mark each TaqMan
1
4. For each array card, pipet 100 µL of the appropriate calibration solution into each of the eight
reservoirs in the array card.
See "Load the PCR reaction mix"
5. Centrifuge the array cards, then check each array card for the correct fill.
See "Centrifuge the card"
6. Seal the array cards.
See "Seal the card"
IMPORTANT! If an array card is sealed improperly, it can leak and contaminate the sample block
and/or it can cause the associated calibration or RNase P test to fail.
IMPORTANT! After the array cards are sealed, store them in a dark place. Do not expose the array
cards to light until you are ready to use them. The dyes in the array cards are photosensitive. Prolonged
exposure to light can diminish the fluorescence of the dye.
QuantStudio ™ 6 Pro Real-Time PCR System and QuantStudio
™
Array Cards from their box and place them on a clean, dry surface.
1
™
Array Card
on page 204.
on page 204.
on page 206.
™ 7 Pro Real-Time PCR System User Guide
Chapter 7 Calibrate and verify instrument performance
on page
™
• VIC
• FAM ™ ™
/ROX
™ ™
• VIC
/ROX
™ Array Card block
85).
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