Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual page 17

™
x1-m1—FAM
1
™
x2-m2—VIC
2
™
x3-m3—ABY
3
Custom dyes
The instrument can run assays designed with custom dyes. Custom dyes include the following options:
• Dyes that are not supplied by Thermo Fisher Scientific.
• Dyes or formulations of dyes that are not system dyes for the instrument.
To use a custom dye on the instrument, review the following requirements.
• Calibrate the instrument for the custom dye (see "Calibrate custom dyes"
• Ensure that the custom dye excites between 455–672 nm and emits between 505–723 nm.
• Select a custom dye that does not overlap with other dyes used in the run (see "System dyes"
page 16).
• The custom dye for use in calibration must be attached to the 5' end of a short DNA
oligonucleotide consisting of the first two bases of the probe sequence without a quencher at
the 3' end.
QuantStudio ™ 6 Pro Real-Time PCR System and QuantStudio
x1-m1 x2-m2 x3-m3 x4-m4 x5-m5 x6-m6
1 2 3 4 5
™
dye, SYBR GREEN dye
dye
™ ®
dye, NED dye, Cy 3, TAMRA
x4-m4—JUN
4
x5-m5—Cy
5
™
dye
™ 7 Pro Real-Time PCR System User Guide
Chapter 1 Product information
Instrument hardware description
Wavelength
(nm)
Emission
Spectra
Filters
™ ™
dye, ROX dye, Texas Red
® ™
5, MUSTANG PURPLE dye
on page 113).
1
™
dye
on
17