Overview Of Data Collection; Veriflex ™ Zones - Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual

Chapter 1 Product information
1
Instrument hardware description

Overview of data collection

The instrument collects raw fluorescence data at different points during the PCR cycle, depending on
the type of run performed.
When you create a plate file, you can customize the optical filter channels through which the instrument
collects data. You can specify a filter channel set for all PCR thermal protocols and for melt curve
stages.
Run type
• Standard curve
Real-time
• Relative standard curve
• Comparative C
• Melt curve
• Genotyping
Post-PCR
(endpoint)
• Presence/absence
VeriFlex ™ Zones
™
VeriFlex Zones provide independent temperature zones that offer enhanced functionality and precise
control over your real-time PCR runs.
The independent zones are ideal for real-time PCR optimization. They also provide the ability to perform
multiple runs at the same time. Unlike standard gradients which give a sigmoidal temperature curve
across the columns, blocks with VeriFlex
The QuantStudio
Pro System has six VeriFlex
™
Note: VeriFlex
18
QuantStudio
Run type
(DDC )
t t
™
6 Pro System has three VeriFlex
™
Zones (Figure 2
Zones are only applicable for the 96-well 0.2-mL block and the 96-well 0.1-mL block.
™ 6 Pro Real-Time PCR System and QuantStudio
During the thermal cycling protocol.
Typical timing is to collect data at each cycle of a PCR
stage or continuously during a melt stage.
• After thermal cycling is completed.
For Presence/Absence and Genotyping analysis,
data collection before the PCR cycle is optional, but
recommended.
• (Optional) During the thermal cycling protocol.
Collecting data during the run can confirm
genotyping results by viewing traces in allelic
discrimination plots or viewing genotyping calls at
earlier cycles.
™
Zones help deliver accurate temperatures across every zone.
™
Zones (Figure 1
on page 19).
Data collection point
on page 19). The QuantStudio
™ 7 Pro Real-Time PCR System User Guide
™
7