Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual page 92

Chapter 7 Calibrate and verify instrument performance
7
Perform ROI/uniformity, background, and dye calibrations for plate blocks
Note: You will be prompted to perform a background calibration after a ROI/Uniformity calibration.
3. (System dye calibration only) Select the calibration plate.
4. Follow the instructions on the screen to start the calibration.
5. Load the plate into the instrument.
CAUTION! The instrument should be used by trained operators who have been warned of the
moving parts hazard.
6. Tap Start.
When the ROI/Uniformity run is complete, you will be prompted to perform a background
calibration.
When the Background calibration is complete, the Calibration Status will display. It will indicate
whether the calibration passed or failed.
7. (For calibrations that pass) Select an option.
Option
Details
Transfer EDS
Reject results
Accept results
If the results are accepted and the calibration file is saved, previous calibration files associated with
the combination of the instrument and the block will no longer be used.
8. (For calibrations that fail) Select an option.
Option
Details
Transfer EDS Transfer the calibration file to Connect, a USB drive, or a network drive (recommended
Close
See "Troubleshoot calibration failure"
9. Unload the plate from the instrument.
CAUTION! PHYSICAL INJURY HAZARD. During instrument operation, the plate temperature
can reach 100°C. Allow it to cool to room temperature before handling.
Note: Use Smart Help if the instrument does not eject the plate (see "Request technical support
with Smart Help"
92
QuantStudio
View the calibration images.
Transfer the calibration file to Connect, a USB drive, or a network drive.
The calibration file is deleted.
The calibration file is saved.
View the calibration images.
for troubleshooting).
The calibration file is deleted.
on page
on page 172).
™ 6 Pro Real-Time PCR System and QuantStudio
Description
Description
95.
™ 7 Pro Real-Time PCR System User Guide