Features Of Each Instrument; Instrument Filters And Supported Dyes - Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual

Chapter 1 Product information
1
Instrument hardware description
• Smart Help to request technical support or instrument service directly from the instrument
• Barcode scanner for tracking plates or TaqMan

Features of each instrument

Feature
[1]
Filter set
™
VeriFlex Zones
TaqMan ™ Array Card
block
Security, Auditing, and
E‑signature (SAE) v2.0
module
[1]
See "System dyes"
[2]
See "VeriFlex ™ Zones"
[3]
See Chapter 9, "Use the instrument with the Security, Auditing, and E‑signature (SAE) v2.0 module".

Instrument filters and supported dyes

System dyes
The QuantStudio
The QuantStudio
For more information about available spectral dye calibration kits, contact Support.
Peak filter Color
x1-m1
x2-m2 Green
x3-m3 Yellow
x4-m4 Orange
x5-m5
x6-m6 Deep-Red
[1]
The central wavelengths are the optimized wavelengths.
[2] ™
The HEX dye and TET
can be added and adapted for use on the instrument.
[3]
This filter set currently does not support any dyes supplied by Thermo Fisher Scientific.
16
QuantStudio
QuantStudio
Coupled five-color filter set
[2]
Three VeriFlex
on page 16.
on page 18.
™
6 Pro Real-Time PCR System has a coupled five-color filter set.
™
7 Pro Real-Time PCR System has a decoupled six-by-six color filter set.
Filter wavelength (nm)
Excitation
Blue 470 ± 15
520 ± 10
550 ± 11
580 ± 10
Red 640 ± 10
662 ± 10
™
dye from Thermo Fisher Scientific fall within the emission wavelength range of the system, therefore they
™ 6 Pro Real-Time PCR System and QuantStudio
™
Array Cards
™
6 Pro System
™
Zones
—
—
[1]
System dyes
Emission
™
FAM
520 ± 15
GREEN
558 ± 11 VIC
™
NED
586 ± 10
dye, ABY
™
623 ± 14 ROX dye, JUN
MUSTANG PURPLE
682 ± 14
dye, Cy
711 ± 12
™
QuantStudio 7 Pro System
Decoupled six-by-six color filter set
™
Six VeriFlex Zones
Available
[3]
Available
Example custom dyes
dye, SYBR
™
dye
™
JOE
™ dye
TET
™
dye, TAMRA
™
dye
™
dye Texas Red
™
®
5
[3]
None Cy
™ 7 Pro Real-Time PCR System User Guide
SYT09
™
dye, HEX dye,
™ [2]
dye
®
Cy 3 dye
™
dye
LIZ ™ dye
®
5.5 dye