Method Elements; Edit The Method - Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual

Method elements

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Method tab
1
Heated cover temperature
2
Reaction volume
3
Stage of thermal protocol
4
Step within a stage
5

Edit the method

1. In the Method tab, tap Edit.
2. Tap a field, then enter the changes.
• Cover temperature
• Volume
• Temperature for any steps
• Time for any steps
• Number of PCR cycles
3. Tap Save.
4. Enter a name for the plate file, then tap Done.
5. In the Save Template screen, select a storage location for the plate file, then tap Done.
A confirmation is displayed.
6. Tap Close.
QuantStudio ™ 6 Pro Real-Time PCR System and QuantStudio
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Chapter 4 Edit a plate before starting a run
Options to edit the method of a plate file
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8
9
Number of cycles for the stage
6
Temperature for the step
7
Hold time for the step
8
Data collection point
9
™ 7 Pro Real-Time PCR System User Guide
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