Perform Roi/Uniformity, Background, Dye, And Normalization Calibrations For The Array Card Block; Calibration Workflow - Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual

Perform ROI/uniformity, background, dye, and normalization calibrations for the TaqMan
Perform ROI/uniformity, background, dye, and
normalization calibrations for the TaqMan
block
To calibrate the plate blocks, see "Perform ROI/uniformity, background, and dye calibrations for plate
blocks" on page
This section covers procedures specific to the TaqMan
for additional information:
• Transfer calibration results
• Transfer data files from calibrations
• Troubleshoot calibration failure
To troubleshoot normalization calibration, see "Troubleshoot normalization calibration failure"
page 102.
• Identify contamination

Calibration workflow

Perform any time that ROI/uniformity and background calibrations are current.
(Optional) Perform custom dye calibrations (see "Calibrate custom dyes"
Perform any time that ROI/uniformity and background calibrations are current.
QuantStudio ™ 6 Pro Real-Time PCR System and QuantStudio
89.
(page 94)
(page
(page
(page 95)
Perform an ROI/uniformity calibration
You are automatically prompted to perform background calibration.
Perform a background calibration
Perform any time that ROI/uniformity calibrations are current.
Perform system dye calibrations
Perform normalization calibration
Perform after a dye calibration.
Chapter 7 Calibrate and verify instrument performance
™
Array Card block. See the following sections
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95)
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™ 7 Pro Real-Time PCR System User Guide
™ Array Card block
™
Array Card
on page 113)
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on
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