Configure Ramp Rates; Add Or Remove A Pause; Options To Edit The Well Details Of A Plate File - Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual

Chapter 4 Edit a plate before starting a run
4

Options to edit the well details of a plate file

Configure ramp rates

The ramp rate is how quickly the temperature changes from one stage or step to another stage or step.
The maximum ramp rate for the instrument is 6.5°C/second.
1. In the Method tab, tap Edit4Advanced4Ramp rates.
Note: Use
2. Tap a ramp rate, then edit the value.
3. Tap Done.

Add or remove a pause

A pause allows you to open the drawer to add additional reagents to the plate.
1. In the Method tab, tap Edit4Advanced4Add pause.
Note: Use
2. Tap
(Pause).
3. Enter a temperature and the cycle.
A pause in the thermal cycling protocol is denoted with a
temperature of the pause are also denoted.
4. Tap
(Pause) to remove the pause.
5. Tap Done.
Options to edit the well details of a plate file
The following files can be edited:
• A system template (see
A system template that is edited must be saved as a plate file. A system template cannot be
overwritten.
• A saved plate file (see "Create and run a plate from a system template or a saved plate file"
page 47)
• The plate file associated with the last run (see "Repeat the last run"
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QuantStudio
or to navigate through the method if it is not all displayed.
or to navigate through the method if it is not all displayed.
page 47)
™ 6 Pro Real-Time PCR System and QuantStudio
. The cycle of the pause and the
on page 49)
™ 7 Pro Real-Time PCR System User Guide
on