Performance Specifications Pass Criteria; Prepare An Rnase P Plate - Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual

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Figure 14 384-well RNase P plate
NTC (no template control)
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STD 1,250 copies
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STD 2,500 copies
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STD 5,000 copies
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Performance specifications pass criteria

After the run, the software calculates average copy number values and standard deviation values.
The instrument passes performance specifications if the following inequality is true and the instrument
successfully distinguishes between unknown populations A and B with a statistical confidence level
of 99.7%.
[(C ) – 3(σ )] > [(C
qA CqA
where:
• C
= Average C
qA
• σ
= Standard deviation of unknown population A
CqA
• C = Average C
qB
• σ
= Standard deviation of unknown population B
CqB
The software automatically adjusts the threshold and omits a defined number of wells from the
unknown populations to meet the performance specifications. To view any omitted wells, open the
data file for the verification in the desktop software.

Prepare an RNase P plate

Materials required for RNase P plate preparation
• RNase P instrument verification plate
• Centrifuge with plate adapter; buckets cleaned before use
• Powder‐free gloves
• Safety glasses
QuantStudio ™ 6 Pro Real-Time PCR System and QuantStudio
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) + 3(σ )]
qB CqB
of unknown population A
q
of unknown population B
q
Chapter 7 Calibrate and verify instrument performance
Perform verification using RNase P plates
STD 10,000 copies
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STD 20,000 copies
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Unknown A (5,000)
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Unknown B (10,000)
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™ 7 Pro Real-Time PCR System User Guide
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