Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual page 118

Chapter 7 Calibrate and verify instrument performance
7
Calibrate custom dyes
Add a custom dye to the instrument
1. In the home screen, tap
The Custom Dye screen is displayed.
2. Tap Add custom dye.
3. Tap the Custom Dye Name field, then enter a name.
IMPORTANT!
·
Do not use a system dye name for a custom dye name.
· Dye names are spacing sensitive and cannot contain special characters.
4. Select the type of dye.
Option
Reporter
Quencher The dye suppresses the fluorescence of a reporter dye until amplification of PCR product.
Both
5. Tap Save.
IMPORTANT! The custom dye must also be added to the desktop software library before creating,
running, or analyzing experiments that use custom dyes.
Perform a custom dye calibration
1. In the instrument home screen, tap
service4Calibration4Custom dye.
The Custom Dye screen is displayed.
2. Tap a custom dye.
3. Review the information, then tap Update.
4. Enter the calibration temperature.
Note: The default calibration temperature is set to 60°C.
5. (Optional) Tap Reagents, then enter the reagent information.
6. Load the plate into the instrument, then tap Start.
CAUTION! The instrument should be used by trained operators who have been warned of the
moving parts hazard.
Calibration complete is displayed when the run is complete.
118
QuantStudio
(Settings)4Maintenance and service4Calibration4Custom dye.
The dye works in conjunction with a quencher dye to report an increase of PCR product.
The dye can be used as a reporter dye or a quencher dye.
™ 6 Pro Real-Time PCR System and QuantStudio
Description
(Settings)4Maintenance and
™ 7 Pro Real-Time PCR System User Guide