Troubleshoot Normalization Calibration Failure - Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual

Chapter 7 Calibrate and verify instrument performance
7
Perform ROI/uniformity, background, dye, and normalization calibrations for the TaqMan
• Calibration name
• Result
• Date
• Whether this calibration is current
4. Tap Detail.
5. (Optional) For Uniformity, Background, and Dye calibration images, tap on an individual well on the
right side to see the results for a single well.

Troubleshoot normalization calibration failure

Normalization calibration is only required for the TaqMan
troubleshooting, see "Troubleshoot calibration failure"
Observation
Normalization calibration failed
102
QuantStudio
Possible cause
Abnormally low spectra across
all wells of the array card.
™ 6 Pro Real-Time PCR System and QuantStudio
™ Array Card block
• The operator
• Reagent name, part number, lot number,
and expiration date
• Comments.
™
Array Card. For all other calibration
on page 95.
Recommended action
CAUTION!
HAZARD. During instrument
operation, the TaqMan
temperature can reach 100°C. Allow
it to cool to room temperature before
handling.
1. Confirm that you loaded the correct array
card into the instrument. If not, perform
the calibration again using the correct
consumable.
2. If you are using the correct array card,
perform the calibration again using a
different normalization consumable.
3. If the calibration fails again, contact
Support.
™ 7 Pro Real-Time PCR System User Guide
PHYSICAL INJURY
™
Array Card