Troubleshoot Verification Failure - Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual

5. When the run is complete and the screen displays Complete, tap View results to confirm the
status of the run.
Verification status
Passed
Failed
6. In the RNase P Verification Status screen, tap one of the following options.
Accept results
Reject results
Export results
7. Unload the plate from the instrument.
CAUTION! PHYSICAL INJURY HAZARD. During instrument operation, the plate temperature
can reach 100°C. Allow it to cool to room temperature before handling.
Note: Use Smart Help if the instrument does not eject the plate (see "Request technical support
with Smart Help"
The status of the RNase P verification is displayed on the Status and Reminders screen (see "View the
calibration status on the instrument"
IMPORTANT! RNase P plates can only be used one time. Do not reuse the RNase P plate.

Troubleshoot verification failure

Observation
Verification failed
QuantStudio ™ 6 Pro Real-Time PCR System and QuantStudio
Instrument is ready for use.
See "Troubleshoot verification failure"
Option
Save the results to the instrument.
Deletes the RNase P verification results.
Exports the results.
on page 172).
on page
Possible cause
The plate was improperly
prepared.
Chapter 7 Calibrate and verify instrument performance
Perform verification using RNase P plates
Action
on page
Description
87).
Ensure the following:
• The correct plate was used for the
verification performed.
• The plate was properly thawed.
• The plate was properly centrifuged.
• The plate was properly sealed.
Open the data file for verification in the desktop
software or the Connect platform to view the
flags and the troubleshooting details for the
failed wells.
™ 7 Pro Real-Time PCR System User Guide
107.
Recommended action
7
107