Rnase P Instrument Verification Taqman ™ Array Card - Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual

The instrument and block require valid ROI/uniformity, background, normalization, and dye calibrations
to perform verification.
For a description of verification, see "Instrument verification description"
For a description of the pass criteria, see "Performance specifications pass criteria"
RNase P instrument verification TaqMan
™
The ViiA 7 Array Card RNaseP Verification Kit includes one empty array card and eight tubes of
solution. Each tube contains reaction mix (PCR master mix, RNase P primers, and FAM
labeled probe) and a known concentration of human genomic DNA template.
To perform an instrument verification run, each solution is loaded into the empty array card. When
complete, the array card contains five replicate groups of standards (200, 400, 800, 1600, and
3200 copies), two of unknown populations (800 and 1600 copies), and one that serves as a no template
control (NTC).
See Figure 15 on page
Figure 15 RNase P standards layout
Port 1, No Template Control
1
Port 2, Unknown A (800 copies)
2
Port 3, Unknown B (1,600 copies)
3
Port 4, Standard 200 copies
4
Port 5, Standard 400 copies
5
Port 6, Standard 800 copies
6
Port 7, Standard 1,600 copies
7
Port 8, Standard 3,200 copies
8
QuantStudio ™ 6 Pro Real-Time PCR System and QuantStudio
109.
™
Chapter 7 Calibrate and verify instrument performance
Perform verification using RNase P with a TaqMan
™
Array Card
7 Pro Real-Time PCR System User Guide
™ Array Card
on page 104.
on page 105.
™
-MGB dye-
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