Calibration And Verification Schedule - Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual

Calibration and verification schedule

The instrument and block will be calibrated during the installation. To ensure optimal performance,
perform calibrations at the recommended frequency.
Note: After instrument calibration, we recommend performing instrument verification using the
provided RNase P plate.
IMPORTANT! Perform calibrations and instrument runs under the specified environmental conditions
(see "Environmental requirements"
affect the instrument performance and shorten the life span of the instrument components.
To set calibration frequencies and reminders, see one of the following sections:
• View the calibration status on the instrument (see
• View calibration status and set reminders on the Connect platform (see
Calibration
ROI/Uniformity
Background
Dye
Normalization
(TaqMan ™ Array Card block
only)
QuantStudio ™ 6 Pro Real-Time PCR System and QuantStudio
on page 192). Exposure to extreme temperatures can adversely
• Every two years (recommended).
• Always perform new Background and Dye calibrations after an ROI/Uniformity
calibration.
You will be prompted to run a Background calibration after the ROI/Uniformity
calibration is complete.
The ROI/Uniformity calibration and the Background calibration must pass in order
to accept the results. It is recommended to transfer the files if the calibration fails
in order to troubleshoot.
Note: Performing an ROI/Uniformity calibration invalidates all other calibrations.
• Every month (recommended).
• Background calibration can also be performed, as needed:
– To check for contamination (depends on usage and laboratory conditions).
– To obtain the most accurate data for the removal of background
fluorescence.
Note: Performing a Background calibration does not invalidate any other calibration.
• Every two years (recommended).
• During a Dye calibration, only the dyes on the given spectral calibration plate are
calibrated.
Note: Performing a Dye calibration for a given dye plate does not invalidate any other
calibration.
Every two years (recommended).
Chapter 7 Calibrate and verify instrument performance
page 87)
Recommended frequency
™ 7 Pro Real-Time PCR System User Guide

Calibration and verification schedule

page 88)
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