Prepare A Calibration Taqman ™ Array Card - Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual

Chapter 7 Calibrate and verify instrument performance
7
Perform ROI/uniformity, background, dye, and normalization calibrations for the TaqMan
Prepare a calibration TaqMan
Required materials to prepare calibration TaqMan
Unless otherwise indicated, all materials are available through thermofisher.com. "MLS" indicates that
the material is available from
Calibration kit
™
ViiA 7 Array Card Spectral Calibration Kit, includes the following
components:
• Empty TaqMan
• FAM ™
dye
• VIC ™ dye
• ROX ™
dye
• ROI dye mix
• Background buffer
• FAM ™
/ROX
• VIC ™ ™
/ROX
Equipment and consumables
Centrifuge with custom buckets and card holders, one of the following:
• Sorvall ™
centrifuge
• Multifuge ™
• Megafuge ™
™
TaqMan Array Card Sealer
Blank balance TaqMan
Pipette, 200 µL with tips
Permanent marker or pen
Powder‑free gloves
Safety glasses
Fill the calibration TaqMan
For detailed instructions to prepare a TaqMan
preparation of a TaqMan
IMPORTANT! Wear powder-free gloves while creating the calibration array cards.
98
QuantStudio
™ Array Card
fisherscientific.com
Item
™
Array Cards
™
dye
dye
centrifuge
centrifuge
™
Array Cards
™
Array Cards
™ Array Card".
™ 6 Pro Real-Time PCR System and QuantStudio
™ Array Card block
™
Array Card
or another major laboratory supplier.
™
Array Card, see Appendix C, "Detailed procedures for
™ 7 Pro Real-Time PCR System User Guide
Source
4432314
Contact your local sales office
Contact your local sales office
Contact your local sales office
MLS
MLS
MLS
MLS