Chapter 7 Calibrate And Verify Instrument Performance; Instrument And Block Calibration - Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual

7
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Instrument and block calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
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Calibration and verification schedule . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
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Calibration descriptions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
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View the calibration status and set reminders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
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Export a calibration report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
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Perform ROI/uniformity, background, and dye calibrations for plate blocks . . . . . . . . . . . . . . . . . . 89
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Perform ROI/uniformity, background, dye, and normalization calibrations for the
™
TaqMan Array Card block . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
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Perform verification using RNase P plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
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Perform verification using RNase P with a TaqMan
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Calibrate custom dyes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
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Calibrate for a custom melt curve run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120

Instrument and block calibration

The QuantStudio
calibration status applies to the combination of the instrument and the block.
If a block is moved to an instrument that it has not been calibrated on, that combination of block and
instrument needs to be calibrated. This applies even if the block has previously been calibrated on a
different instrument.
If a block is moved back to an instrument that is has previously been calibrated on, it does not need to
be calibrated again. It needs to be calibrated again if the calibration is expired.
A run can still be started if a calibration of the block on the instrument is expired. A run cannot be
started if a block has never been calibrated on the instrument.
84
QuantStudio
Calibrate and verify instrument
™
6 Pro System and the QuantStudio
™ 6 Pro Real-Time PCR System and QuantStudio
performance
™
Array Card . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
™
7 Pro System have interchangeable blocks. The
™ 7 Pro Real-Time PCR System User Guide