Calibrate For A Custom Melt Curve Run - Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual

Chapter 7 Calibrate and verify instrument performance
7

Calibrate for a custom melt curve run

10. Unload the plate from the instrument.
CAUTION! PHYSICAL INJURY HAZARD. During instrument operation, the plate temperature
can reach 100°C. Allow it to cool to room temperature before handling.
Note: Use Smart Help if the instrument does not eject the plate (see "Request technical support
with Smart Help"

Calibrate for a custom melt curve run

Use this calibration to prepare for high resolution melt analysis runs.
Note: A custom melt calibration calibrates a custom dye and a melt calibration at the same time.
• Ensure that all calibrations are current (see "View the calibration status on the instrument"
page 87).
• Add a custom dye (see "Add a custom dye to the instrument"
1. In the home screen, tap
2. Select the option for the kit that you are using.
• PCR + Melt
• Melt only
3. Select or add a dye, then select a filter set appropriate for your dye's wavelength (see filter-
wavelength table below).
Note: See your reagent kit documentation for dye name and wavelength information.
[1]
The central wavelengths are the optimized wavelengths.
IMPORTANT! If the selected filter set does not match your reagent kit documentation, then the
incorrect wavelength may be collected during a run.
4. (Optional) Tap Reagents, then enter the reagent information.
120
QuantStudio
on page 172).
(Settings)4Maintenance and service4Calibration4Custom melt.
Peak filter
x1-m1
x2-m2
x3-m3
x4-m4
x5-m5
x6-m6
™ 6 Pro Real-Time PCR System and QuantStudio
on page
Filter wavelength (nm)
Excitation
470 ± 15
520 ± 10
550 ± 10
580 ± 10
640 ± 10
662 ± 10
™ 7 Pro Real-Time PCR System User Guide
on
118).
[1]
Emission
520 ± 15
558 ± 12
587 ± 10
623 ± 14
682 ± 14
711 ± 12