Appendix C Detailed Procedures For Preparation Of A Taqman; Array Card; Guidelines For Preparation Of A Card - Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual

C
Detailed procedures for preparation

Guidelines for preparation of a card

• Keep the card protected from light and stored as indicated until ready for use. Excessive exposure
to light may affect the fluorescent probes of the dried-down assays in the card.
• Before removing the card from its packaging:
– Prepare each sample-specific PCR reaction mix.
– Allow the card to reach room temperature.
• Load each fill reservoir with 100 µL of sample-specific PCR reaction mix.
– Each fill reservoir contains a single sample as determined by the card layout.
– The 100–µL volume ensures adequate filling of each reaction well. Volumes smaller than
100 µL result in insufficiently filled cards.
• Do not allow the micropipette tip to contact the coated foil beneath the fill port.
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QuantStudio
of a TaqMan
™ 6 Pro Real-Time PCR System and QuantStudio
™

Array Card

™ 7 Pro Real-Time PCR System User Guide