Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual page 208

Appendix C Detailed procedures for preparation of a TaqMan
C
Seal the card
IMPORTANT!
·
Do not use excessive force or speed when pushing the carriage across the card.
·
Do not move the carriage back across the card. Leave the carriage at the ending position while
removing the card from the sealer.
4. Remove the sealed card from the sealer by grasping the sides of the card and lifting it off.
Use the thumb slot in the middle of the sealer to access the card.
5. Examine the card for proper sealing.
Note: When properly sealed, the indentations from the sealer carriage align with the main
channels of the card.
If the indentations do not align or if the foil is damaged, do not use the card.
6. Use scissors to trim the fill reservoir strip from the card. Use the edge of the card carrier as a
guide.
IMPORTANT! Completely remove the fill reservoir strip. Any remaining plastic that extends
beyond the card edge can prevent the card from seating properly on the sample block and can
affect amplification.
The card is now ready to run on the instrument.
(Optional) Store the sealed card, protected from light, at 2−8°C. The length of time the card can be
stored depends on the master mix. See the user guide for the assay or the master mix.
208
QuantStudio
Correct trim
™ 6 Pro Real-Time PCR System and QuantStudio
™ Array Card
Incorrect trim
™ 7 Pro Real-Time PCR System User Guide