Calibration Workflow; Prepare A Calibration Plate - Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual

Chapter 7 Calibrate and verify instrument performance
7
Perform ROI/uniformity, background, and dye calibrations for plate blocks

Calibration workflow

Perform any time that ROI/uniformity and background calibrations are current.
(Optional ) Perform custom dye calibrations (see "Calibrate custom dyes"
Perform any time that ROI/uniformity and background calibrations are current.

Prepare a calibration plate

Required materials to prepare calibration plates
Note: We recommend calibrating with all Spectral Dye Calibrations Plates available for your block
configuration even if you are not using all the dyes in the plates. This is recommended for optimal
system performance.
Unless otherwise indicated, all materials are available through thermofisher.com. "MLS" indicates that
the material is available from
Calibration plates
ROI/Uniformity plate
Background calibration plate
Dye calibration plates
Equipment and consumables
Centrifuge with plate adapter; buckets cleaned before use
Powder‑free gloves
Safety glasses
90
QuantStudio
Perform an ROI/uniformity calibration
You are automatically prompted to perform background calibration.
Perform a background calibration
Perform any time that ROI/uniformity calibrations are current.
Perform system dye calibrations
fisherscientific.com
Item
™ 6 Pro Real-Time PCR System and QuantStudio
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or another major laboratory supplier.
™ 7 Pro Real-Time PCR System User Guide
on page 113)
Source
MLS
MLS
MLS