Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual page 205

d. Place the loaded card holder into the bucket so that the card holder label faces the front of
the bucket.
2. Configure the centrifuge using its front‑panel controls.
a. Set the bucket type to 15679.
b. Set the following parameters according to the control panel type.
Increasing ramp rate
Decreasing ramp rate
Rotational speed
Centrifugation time
[1]
You will centrifuge the cards twice, each time for 1 minute (see step 4).
IMPORTANT! A speed that is set too high can deform the card.
3. Load the buckets into the centrifuge.
a. Press
the centrifuge cover.
b. Place each loaded bucket onto an open rotor arm
of the centrifuge.
Ensure that each bucket can swing easily within
its slotted position on the rotor arm.
c. If there are empty rotor arms, prepare buckets
with blank balance cards as described in step 1.
Place the balance buckets onto the rotor arms.
The rotor must be evenly loaded and opposing
buckets must hold the same weight.
d. Close the centrifuge cover.
QuantStudio ™ 6 Pro Real-Time PCR System and QuantStudio
Appendix C Detailed procedures for preparation of a TaqMan
►
Parameter EASYSet (touchpad-operated)
[1]
on the centrifuge control panel to open
9
9
1,200 rpm (331 × g)
1 minute
Centrifuge is properly loaded and balanced.
™ 7 Pro Real-Time PCR System User Guide
™ Array Card
Centrifuge the card
QUIKSet (knob-operated)
3
—
1,200 rpm
1 minute
C
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