Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual page 119

7. Tap View results4Details.
8. Review the plot.
Calibration results that pass show uniform signals with peaks that are aligned with the dye
wavelength.
[1]
The central wavelengths are the optimized wavelengths.
Figure 17 An example of a dye calibration plot
The peaks for your dye may align with a different filter set.
9. Select an action depending on whether the custom dye calibration passed or failed.
Calibration
status
Passed
Failed
QuantStudio ™ 6 Pro Real-Time PCR System and QuantStudio
Peak filter
x1-m1
x2-m2
x3-m3
x4-m4
x5-m5
x6-m6
1. Tap Accept Results or Reject Results.
Note: Accepting the results saves the calibration data to the instrument and
overwrites existing data.
2. (Optional) Tap Transfer to EDS to transfer the calibration data to a USB drive.
1. Perform the calibration again using a new custom dye plate. Create the plate
using the next dye concentration greater than the optimal dye concentration (see
"Determine the optimal dye concentration"
2. For more information about calibration failures, see "Troubleshoot calibration
failure" on page
Chapter 7 Calibrate and verify instrument performance
Filter wavelength (nm)
Excitation
470 ± 15
520 ± 10
550 ± 11
580 ± 10
640 ± 10
662 ± 10
Action
95.
™ 7 Pro Real-Time PCR System User Guide
Calibrate custom dyes
[1]
Emission
520 ± 15
558 ± 11
586 ± 10
623 ± 14
682 ± 14
711 ± 12
on page 116).
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