Chapter 7 Calibrate and verify instrument performance
7
Perform ROI/uniformity, background, and dye calibrations for plate blocks
(continued)
Calibration
Background
Dye
5. (Optional) For Uniformity, Background, and Dye calibration images, tap on an individual well on the
right side to see the results for a single well.
Transfer calibration results
1. In the home screen, tap
reminders.
The Status and Reminders screen is displayed.
2. Tap a calibration type in the table.
3. Tap Export.
Transfer calibration data files
This section describes transferring calibration files if the calibration passed.
If the calibration fails, the calibration data file is not saved. The data file can only be transferred
immediately after the calibration (see "Perform calibrations"
1. In the home screen, tap
reminders.
The Status and Reminders screen is displayed.
2. Tap a calibration type in the table, then tap View.
3. Tap a calibration date in the table, then tap View.
4. Tap Transfer EDS.
94
QuantStudio
Example results indicating successful calibration
Few, if any, signals with abnormally high fluorescence.
Signals from each well following a uniform trend, and each dye
peaks at the correct filter.
(Settings)4Maintenance and service4Calibration4Status and
(Settings)4Maintenance and service4Calibration4Status and
™
6 Pro Real-Time PCR System and QuantStudio
on page
91).
™
7 Pro Real-Time PCR System User Guide
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