Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual page 116

Chapter 7 Calibrate and verify instrument performance
7
Calibrate custom dyes
Run the dilution plate
IMPORTANT! Do not run the dilution plate as a calibration.
1. Load the plate into the instrument.
CAUTION! The instrument should be used by trained operators who have been warned of the
moving parts hazard.
2. Set up a genotyping plate file on the instrument (see "Create and run a plate from a system
template or a saved plate file"
To use a system template for genotyping, see "System template file names"
genotyping method that is compatible with the Master Mix.
3. In the Method tab, set the hold to 60°C for 2 minutes.
4. In the Plate tab, enter the dilution series information for the appropriate wells.
5. Tap Start run.
6. When the run is complete, transfer the data files for analysis (see "Transfer a data file immediately
after a run"
7. Unload the plate from the instrument.
CAUTION! PHYSICAL INJURY HAZARD. During instrument operation, the plate temperature
can reach 100°C. Allow it to cool to room temperature before handling.
Note: Use Smart Help if the instrument does not eject the plate (see "Request technical support
with Smart Help"
Determine the optimal dye concentration
Use the Connect platform or the desktop software to review the dye signal data and select the dilution
to use for dye calibration.
1. In the Results tab, select Raw data plot.
This plot displays the raw fluorescence signal of each optical filter, for individual wells.
Note: The raw data plot cannot be viewed on the instrument touchscreen.
2. For each replicate population of dilutions, select the wells in the plate layout to view in the plot.
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7 Pro Real-Time PCR System User Guide