Thermo Scientific Applied Biosystems QuantStudio 6 Pro User Manual page 121

5. Load the plate into the instrument
6. Tap Start.
7. When the run is complete and the screen displays Calibration complete, tap View
results4Details.
8. Review the plot.
Passing calibration results show uniform signals with peaks that are aligned with the dye
wavelength.
Figure 18 An example dye calibration plot
Note: The peaks for your dye may align with a different filter set.
9. Select an action depending on whether the custom dye calibration passed or failed.
Calibration
status
Passed
Failed
10. Unload the plate from the instrument.
Note: You must also add the custom dye to the desktop software dye library before you create, run, or
analyze experiments that use custom dyes.
Note: To perform a custom melt experiment, you can either create a Standard Curve or a Custom
experiment with melt, then specify the data points per degree in the method.
QuantStudio ™ 6 Pro Real-Time PCR System and QuantStudio
1. Tap Accept Results or Reject Results.
Note: Accepting the results saves the calibration data to the instrument and
overwrites existing data.
2. (Optional) Tap Transfer EDS to transfer the calibration data to a USB drive.
1. Perform the calibration again using a new custom dye plate. Create the plate
using the next dye concentration greater than the optimal dye concentration (see
"Determine the optimal dye concentration"
2. For more information, see "Troubleshoot calibration failure"
™
Chapter 7 Calibrate and verify instrument performance
Calibrate for a custom melt curve run
Action
on page
7 Pro Real-Time PCR System User Guide
116).
on page 95.
7
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