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E-Gel
Power Snap Electrophoresis System
™
USER GUIDE
E-Gel
Power Snap Electrophoresis Device and E-Gel
™
For use with E-Gel
™
SizeSelect
agarose gels
™
Catalog Numbers G8100, G8200, G8300, G8141ST, G8142ST, G8151ST, G8152ST, G8168ST,
G8162ST, G8341ST, G8342ST, G8351ST, G8352ST and A33811
Publication Number MAN0017050
Revision B.0
For Research Use Only. Not for use in diagnostic procedures.
, E-Gel
EX, E-Gel
™
™
Power Snap Camera
™
Go!, CloneWell
, and
™
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Table of Contents
Troubleshooting
Related Manuals for Thermo Scientific Invitrogen E-Gel G8100
Summary of Contents for Thermo Scientific Invitrogen E-Gel G8100
- Page 1 E-Gel Power Snap Electrophoresis System ™ USER GUIDE E-Gel Power Snap Electrophoresis Device and E-Gel Power Snap Camera ™ ™ For use with E-Gel , E-Gel EX, E-Gel Go!, CloneWell , and ™ ™ ™ ™ SizeSelect agarose gels ™ Catalog Numbers G8100, G8200, G8300, G8141ST, G8142ST, G8151ST, G8152ST, G8168ST, G8162ST, G8341ST, G8342ST, G8351ST, G8352ST and A33811 Publication Number MAN0017050...
- Page 2 Thermo Fisher Scientific Baltics UAB | V.A. Graiciuno 8, LT-02241 | Vilnius, Lithuania For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition. The information in this guide is subject to change without notice. DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
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Page 3: Table Of Contents
Contents About this guide ........................7 Purpose of the guide ......................7 Safety ..........................7 Product Information ......................8 Product description ........................ 8 Features ..........................8 Throughput ........................8 System components......................8 Kit contents and storage......................9 Upon receiving the instrument....................9 Storage .......................... - Page 4 Home screen ........................21 Attach the camera .........................22 Remove the camera.......................22 View gel ..........................23 Capture image ........................23 Adjust capture settings......................23 Automatic image capture......................24 Cancel auto capture ......................24 Export image ........................24 Export from capture screen ....................24 Export from image gallery ....................24 E-Gel CloneWell II gels....................
- Page 5 Upgrade system firmware ......................37 Battery replacement ......................38 Instrument Specifications .......................38 Instrument dimensions and specifications ................38 Electrical requirements.......................39 Environmental requirements....................39 Appendix C.......................... 40 E-Gel agarose gels ......................40 ™ Choosing the right gel ......................41 Analytical gels ........................41 Gels for preparative gel electrophoresis in Cloning and NGS applications ........41 Other available gel types for routine electrophoresis..............42 Opening E-Gel cassettes .......................42...
- Page 6 Instrument starter kits......................50 E-Gel agarose gels ......................52 ™ Accessory products .......................52 Accessory items........................53 Appendix H ......................... 54 Safety ..........................54 Before starting........................54 Installing the instrument .....................55 Electromagnetic compatibility (EMC) standards .................55 Class A notice ........................55 Electrical safety ........................56 Service operation requirements....................56 LED (Light-Emitting Diode) ....................56 Explanation of symbols and warnings ..................57 Appendix I ...........................
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Page 7: About This Guide
About this guide Important: Before using this product, read and understand the information in the “Safety” appendix in this document. Purpose of ™ This user guide contains detailed information about usage of the E-Gel Power Snap ™ Electrophoresis System and E-Gel pre-cast agarose gels. -
Page 8: Product Information
Product Information Product description ™ The E-Gel Power Snap Electrophoresis System is designed to produce a fast and convenient DNA agarose gel electrophoresis and documentation workflow. The E-Gel ™ Power Snap Electrophoresis System is composed of two units: The E-Gel ™... -
Page 9: Kit Contents And Storage
Kit contents and storage Depending on the ordered catalog number the product will arrive with following components: Component G8100 G8200 G8300 E-Gel Power Snap Electrophoresis Device 1 each — 1 each ™ E-Gel Power Snap Camera — 1 each 1 each ™... -
Page 10: Description Of Parts
Description of parts Front view E-Gel Power Snap Camera ™ (docked) E-Gel Power Snap ™ Electrophoresis Device Camera control touch screen USB port for image export/firmware upgrade Electrophoresis unit control touch screen ... -
Page 11: Parts Of The E-Gel ™ Power Snap Electrophoresis Device
Parts of the E-Gel Power Snap Electrophoresis Device ™ USB port for firmware upgrade Adaptor DC input DC output cable Power switch Connector to DC input of electrophoresis unit ... -
Page 12: Parts Of The E-Gel ™ Power Snap Camera
Parts of the E-Gel Power Snap Camera ™ Docking connector cover (open) Docking connector Camera connector Battery compartment USB port for image export/firmware upgrade E-Gel Power Snap Electrophoresis System User Guide ™... -
Page 13: User Graphical Interface Overview
User graphical interface overview ™ ™ The E-Gel Power Snap Electrophoresis System is intuitive and easy-to-use. Both the E-Gel Power Snap Electrophoresis Device and E-Gel ™ Power Snap Camera are controlled using touch screens. The following table describes common controls of the Power Snap system. Control Function E-Gel... -
Page 14: Using The E-Gel ™ Power Snap Electrophoresis Device
Using the E-Gel Power Snap Electrophoresis Device ™ ™ This section provides instructions for performing electrophoresis using the E-Gel Power Snap Electrophoresis Device. For specific protocols describing the use of E-Gel ™ ™ II Agarose Gels, see page 25. CloneWell For specific protocols describing the use of E-Gel ™... -
Page 15: Dilute Samples Containing High Salt
Dilute E-Gel ™ EX gels are sensitive to high salt and EDTA content. Samples containing ≥50 mM NaCl, 100 mM KCl, 10 mM acetate ions, or 10 mM EDTA (i.e., certain restriction enzyme and PCR samples buffers) cause loss of resolution on E-Gel ™... -
Page 16: Prepare Gel
Prepare gel Remove E-Gel ™ agarose gel from package. Gently remove comb from the cassette. Load the gel into the cassette compartment, starting from the right edge. Press down on the left side of the cassette to secure the cassette. Load gels within 15 minutes after opening the package. -
Page 17: Sample Loading Guidelines
Sample loading guidelines • Use the recommended total loading volume for each gel type. Do not load more than recommended amount of DNA sample or ladder per well. • Load deionized water into all empty wells. • Keep all sample volumes uniform. If you do not have enough samples to load all the wells of the gel, load an identical volume of deionized water into any empty wells. -
Page 18: Run The Gel
Run the gel Press Set up run to start E-Gel ™ protocol selection. Select the E-Gel ™ protocol corresponding to your gel type. Use the up/down arrows to navigate through the menu. (Optional) For recurring experiments, select the last used protocol. Gel Type Recommended Default run... -
Page 19: Check Status
(Optional) Adjust the duration of the gel run using the +/– buttons or press in the duration field to open a keyboard to enter a number. Press Start run to begin running the gel. Note: Do not exceed the maximum run time indicated for the specific gel type, as this will impact separation quality. -
Page 20: Cancel The Run
Cancel the Press Pause run to temporarily stop the run. Press the status dial. Press Cancel run to stop the run. Edit gel Press Pause run to temporarily stop the run. duration Press the status dial. Press Edit gel duration. Adjust the protocol duration using the +/–... -
Page 21: Using The E-Gel ™ Power Snap Camera
Using the E-Gel Power Snap Camera ™ General guidelines • ™ ™ The E-Gel Power Snap Camera is an integral part of The E-Gel Power Snap Electrophoresis System, and only works when docked to The E-Gel ™ Power Snap Electrophoresis Device. •... -
Page 22: Attach The Camera
Attach the camera The E-Gel ™ Power Snap Camera can be attached to ™ the E-Gel Power Snap Electrophoresis Device either during a run, or after the run is completed. Unfasten the docking connector cover. Align the docking connector with the camera connector. -
Page 23: View Gel
View gel Press View Gel to access the view gel screen and visualize the bands on the gel. Adjust exposure setting if necessary. Note: The gel image in the capture screen is a still picture which is refreshed periodically, or when adjustment sliders are used. -
Page 24: Automatic Image Capture
Automatic image capture The E-Gel ™ Power Snap Camera can automatically capture images as the gel runs. The camera can capture and save 2–5 images of the gel at evenly spaced intervals. Press Settings / Select Auto capture. Select one of following capture methods: Smart exposure: captures each image at the optimal exposure level. -
Page 25: E-Gel ™ Clonewell ™ Ii Gels
E-Gel CloneWell II gels ™ ™ ™ ™ ™ E-Gel CloneWell II pre-cast agarose gels are designed for use with the E-Gel Power Snap Electrophoresis Device, and provide a fast, safe, and effective DNA fragment isolation method for DNA cloning workflows. Advantages •... -
Page 26: Load Samples
Load samples Fill all wells of both rows with 50 μL of deionized water. Load 25 μL of sample with 1X Sample Loading Buffer into wells from the bottom up. Do not damage the gel or introduce bubbles into the wells. Load 25 μL of ready-to-use E-Gel ™... -
Page 27: Collect Dna Fragment
Collect DNA fragment Resume the run and carefully observe as the band of interest fully enters the recovery well. Stop the gel and recover the sample with a pipette. Avoid piercing the agarose. Some residual DNA will remain visible in the well due to migration into the agarose at the bottom of the well. -
Page 28: Troubleshooting
Troubleshooting For common E-Gel ™ troubleshooting guidelines refer to troubleshooting guide (see page 35). Observation Cause Recommended action Poor resolution or Sample is overloaded Do not load more than 800 ng of DNA in a single smearing of bands lane High salt concentration Dilute your samples 2- to 30-fold Total sample volume is too low... -
Page 29: E-Gel ™ Sizeselect ™ Ii Gels
E-Gel SizeSelect II gels ™ ™ ™ ™ ™ E-Gel SizeSelect II 2% Agarose Gels are designed for use with the E-Gel Power Snap Electrophoresis Device and provide a fast and convenient method for DNA fragment library size selection as part of NGS library preparation workflows. -
Page 30: Load Samples
Load samples Fill all wells of both rows with 50 μL of deionized water. Load 25 μL of sample with 1X Sample Loading Buffer into wells from the bottom up. Do not damage the gel or introduce bubbles into the wells. ™... -
Page 31: Collect Dna Fragment
Collect DNA fragment Resume the run and carefully observe as the reference band enters the recovery well. Important: See NGS library size selection reference to determine when to collect samples of specific target library length. Stop the gel and recover the sample with a pipette. - Page 32 NGS library size selection reference Target Input Stop the run and collect your Schematic Library Size Run time to library sample sample when… view reference amount peak line Ion S5™ XL System 600 bp band has just completely entered the top edge of the 500 ng collection well 600-base-read...
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Page 33: Quantitation Of Isolated Dna
Quantitation of isolated DNA • Recovered DNA can be assessed using the Qubit ™ fluorometer (Cat. no. Q32868), or by gel electrophoresis. • qPCR is recommended for accurate quantitation of next generation sequencing libraries ™ ™ recovered from E-Gel SizeSelect II gels. -
Page 34: Troubleshooting
Troubleshooting For common E-Gel ™ troubleshooting guidelines refer to troubleshooting guide (see page 35). Observation Cause Recommended action Poor resolution or Sample is overloaded Do not exceed 500 ng of total DNA per one sample smearing of bands lane or 500 ng DNA per one band. Do not exceed 1 µg for sheared DNA High salt concentration Dilute your samples 2- to 30-fold depending on the... -
Page 35: Troubleshooting
Appendix A Troubleshooting Observation Cause Recommended action Remove and re-insert cassette or try using new No current Cassette improperly cassette. Use properly stored gels before the specified Inserted, defective or expired expiration date. Incorrect adaptor used Use only UL Listed Class 2 Direct Plug-in Adaptor included with the E-Gel Power Snap Electrophoresis ™... - Page 36 Observation Cause Recommended action Sample leaking from the Sample is overloaded Load the recommended sample volume per well wells Wells damaged during comb Remove the gel comb gently without damaging removal the wells DNA sample cannot be Inhibition of visualization by heat Wait 10–15 minutes for gel to cool before seen visualization...
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Page 37: System Maintenance
Appendix B System maintenance Repeated instrument use can result in formation of spots and smudges on the glass over the transilluminator and on the amber filter, which can then decrease image quality. Clean the glass over the transilluminator and amber filter as needed. -
Page 38: Battery Replacement
Battery replacement The E-Gel ™ Power Snap Camera contains a 3 V CR2450 battery which is required to record the file date and time for the captured images. When battery runs out, the system will indicate the need to replace it. Open the battery compartment on the underside of the E-Gel ™... -
Page 39: Electrical Requirements
Electrical requirements Warning: For safety, the power outlet used for powering the instrument must be accessible at all times. In case of emergency, you must be able to immediately disconnect the main power supply to the instrument. Allow adequate space between the wall and the equipment so the power cord can be disconnected in case of emergency. •... -
Page 40: E-Gel ™ Agarose Gels
Appendix C E-Gel agarose gels ™ E-Gel ™ agarose gels are precast bufferless gels with electrodes embedded in the agarose matrix. Each gel contains an ion generating system, a pH balancing system, and DNA stain packaged inside a transparent plastic cassette. Each gel cassette contains two ion exchange matrices (IEMs) that are in contact with the gel and electrodes. -
Page 41: Choosing The Right Gel
Choosing the right gel To obtain the best results for your application, it is important to choose the correct agarose percentage and well format. The tables below list the various types of gel and resolution for each gel type. Analytical gels E-Gel E-Gel E-Gel... -
Page 42: Other Available Gel Types For Routine Electrophoresis
Other available gel types for routine electrophoresis ™ E-Gel EX Agarose Gels can be used to run RNA samples. RNA can be run under denaturing or non-denaturing conditions. Use non-denaturing conditions only when checking for RNA quality, where accurately determining size is not critical. See page 46 for instructions on performing electrophoresis of RNA samples. -
Page 43: Cleaning And Storage
Cleaning After use, clean the E-Gel ™ Opener with mild detergent and water to remove any excess agarose, and plastic from the platform. Use a squirt bottle and wipe the platform dry with a clean tissue. Do and storage not insert your fingers into the area housing the blades, and do not immerse the E-Gel ™... -
Page 44: Opener
Open the E-Gel cassette with an E-Gel Opener ™ ™ ™ Place the E-Gel Opener on a flat surface, with the knob extending off the edge of the laboratory bench and facing the user. ™ Set the E-Gel Opener to its widest open position by turning the knob counterclockwise. ™... -
Page 45: Choosing The Right Dna Ladder
Appendix D Choosing the right DNA ladder Use the following table to select the E-Gel ™ DNA ladder that yields the best resolution for your E-Gel ™ agarose gel. E-Gel 1 Kb E-Gel 1 Kb E-Gel E-Gel 96 High E-Gel E-Gel Ultra Millenium... -
Page 46: Ex Agarose Gels
Appendix E Running RNA Samples on E-Gel EX Agarose Gels ™ ™ E-Gel EX Agarose Gels can be used to run RNA samples. RNA can be run under denaturing or non-denaturing conditions. Use non-denaturing conditions only when checking for RNA quality, where accurately determining size is not critical. -
Page 47: E-Gel ™ Power Snap Blue-Light Transilluminator
Appendix F E-Gel Power Snap Blue-Light Transilluminator ™ ™ To monitor sample separation right at laboratory bench, the E-Gel Power Snap Electrophoresis Device has an integrated blue-light LED source with emission maximum at 465 nm. This enables real-time monitoring of samples running on E-Gel ™... -
Page 48: Agarose Gels
Nucleic acid stain use in E-Gel agarose gels ™ SYBR Safe DNA Gel Stain ™ ™ SYBR Safe DNA gel stain has been specifically developed for reduced mutagenicity, making it safer than ethidium bromide for staining DNA in agarose gels. The detection sensitivity of E-Gel ™... -
Page 49: Sybr ™ Gold Ii Gel Stain
SYBR Gold II Gel Stain ™ ™ ™ ™ ™ SYBR Gold II gel stain has been specifically developed for E-Gel EX, E-Gel SizeSelect II and E-Gel Go! agarose gels. This gel stain has high sensitivity, with detection down to 0.5 ng/band of DNA. -
Page 50: Instrument Starter Kits
Appendix G Instrument starter kits E-Gel Power Snap Electrophoresis Device Starter Kit Included Equipment ™ E-Gel Power E-Gel Power Cord Safe Imager Cat. No. ™ ™ Gel Knife Snap Power w/ Adaptor Viewing Electrophoresis Snap Glasses (Cat. Device Camera No. S37103) G8141ST 1 each G8142ST... - Page 51 E-Gel Power Snap Electrophoresis Device Starter Kit Included Reagent List ™ E-Gel™ Agarose Gel Ladders Cat. No. Type # of E-Gel™ E-Gel™ E-Gel™ E-Gel™ 96 E-Gel™ Low E-Gel™ Gels 1Kb Plus 1Kb Plus 50 bp High Range Range Sizing DNA Ladder Express Quant.
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Page 52: E-Gel ™ Agarose Gels
E-Gel agarose gels ™ Refer to Choosing the right gel (page 41) to select the most suitable gel for your application. Products % Agarose Quantity Catalog No. 10 gels G401001 20 gels G402001 E-Gel EX Agarose Gels 10 gels G401002 ™... -
Page 53: Accessory Items
Accessory items Product Quantity Catalog No. Safe Imager Viewing Glasses 1 each S37103 ™ Gel Knife 1 each EI9010 E-Gel Opener 1 each G530001 E-G el Power Snap Electroph oresis System User Guide ™... -
Page 54: Safety
Appendix H Safety Before Before you begin using this product, or any installation or service operation, please read the following safety information. Attention to these warnings will help prevent personal injuries and starting damage to the products. It is your responsibility to use the product in an appropriate manner. This product is designed for use solely in laboratory environments, and must not be used in any way that may cause personal injury or property damage. -
Page 55: Installing The Instrument
Installing The product may be installed only under the conditions and in the positions specified by Thermo Fisher Scientific. instrument Following are the required operating position and conditions: • Do not place the product in an area where it will be subject to vibration. •... -
Page 56: Electrical Safety
Electrical safety The following information on electrical safety must be observed, failing to follow these instruction may result in electric shock, fire and/or serious personal injury or death. Service In the event of an equipment malfunction, it is the responsibility of the customer to report the need for service to Thermo Fisher Scientific or to one of the authorized agents. -
Page 57: Explanation Of Symbols And Warnings
Explanation of symbols and warnings The following table explains the symbols displayed on the instrument. Symbol Explanation The CE mark symbolizes that the product conforms to all applicable European Community provisions for which this marking is required. The E-Gel Power Snap Electrophoresis System complies with the Underwriters Laboratories Inc. ™... -
Page 58: Customer And Technical Support
Appendix I Customer and technical support Visit Thermo Fisher Scientific support for the latest in services and support, including: • Worldwide contact telephone numbers • Product support, including: – Product FAQs – Software, patches, and updates • Order and web support •... - Page 59 For support visit thermofisher.com/techresources or email techsupport@lifetech.com thermofisher.com/lifescience 08 April 2020...
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