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This regulation does not apply to reproductions for in-plant use. Trademarks MSC Advantage and SmartFlow are registered trademarks of Thermo Scientific. Thermo Scientific is a brand of Thermo Fisher Scientific Inc. All other trademarks mentioned in the operating instructions are the exclusive property of the respective manufacturers.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Contents 1 General notes ..................6 Instruction of the operating personnel ........... 6 Applicability of the instructions ............7 Warranty ..................7 Explanation of symbols ..............8 1.4.1 Symbols used in the operating instructions ......8 1.4.2...
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Operating Instructions Biological Safety Cabinet | MSC-Advantage Contents 8 Shut-down ..................... 37 Interrupting an operation ............... 37 Shutting the device down .............. 37 9 Cleaning and decontamination ............38 Decontamination procedure ............38 Wipe/spray disinfection ..............38 UV disinfection after a wipe/spray disinfection ......40 9.3.1...
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Operating Instructions Biological Safety Cabinet | MSC-Advantage General notes The following are the addresses of the international Thermo FisherScientific Sales Organisations. Postal address Germany Thermo Electron LED GmbH Robert-Bosch-Straße 1 D - 63505 Langenselbold Inquiries from Germany Phone Phone Sales...
Operating Instructions Biological Safety Cabinet | MSC-Advantage General notes Data of the MSC-Advantage and the documentation Device identification Device Name: Biological Safety Cabinet MSC-Advantage ™ Model: Model lines: 0.9, 1.2, 1.5, 1.8 Model 1.2.from serial devices: 41070608 Model 1.8.from serial devices:...
Operating Instructions Biological Safety Cabinet | MSC-Advantage General notes Warranty Thermo Fisher Scientific warrants the operational safety and functions of the bi- ological safety cabinet only under the condition that: • the device is operated and serviced exclusively in accordance with its intended purpose and as described in these operating instructions, •...
Operating Instructions Biological Safety Cabinet | MSC-Advantage General notes Explanation of symbols 1.6.1 Symbols used in the operating instructions WARNING! is used if non-observance may cause serious or even lethal injuries. CAUTION! is used if non-observance may cause medium to minor injuries or damage.
Operating Instructions Biological Safety Cabinet | MSC-Advantage General notes 1.6.2 Symbols on the device Observe operating instructions (switchbox ceiling) Warning against hand injuries (device side walls) Biohazard (left front section of device) CE Conformity declaration Checked safety (cover light dome) Norme Française / NF - Postes de Sécurité...
Operating Instructions Biological Safety Cabinet | MSC-Advantage General notes Use of the device 1.7.1 Correct use The Biological Safety Cabinet is a laboratory device for installation and operation in microbiological and biotechnical laboratories of safety levels 1, 2, and 3. It has been designed as a Class II microbiological biological safety cabinet, in accordance with EN 12469.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Delivery Scope of delivery Delivery for the biological safety cabinet includes the following: • biological safety cabinet (without stand) • armrests • device documentation: – operating instructions – factory test report Optional components and accessories are listed as separate items in the deli- very document.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Installation Ambient conditions The operational safety and correct function of the device depends on the location where it is to be operated. The biological safety cabinet must be operated only at locations that meet the ambient conditions listed below.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Installation Room ventilation The room ventilation should preferably be a ventilation system that complies with the national requirements for the application. • The inlet air and exhaust air openings of the room ventilation must be located so that drafts are prevented from impairing the function of the safety cabinet air system.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Installation Correct location Choose a draft-free location where the biological safety cabinet does not interfere with the plant traffic. Fig. 1: This figure shows preferred locations for biological safety cabinets and unsuitable locations that are not in accordance with the safety requirements.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Installation Transport Fig. 2: To prevent tilting, always transport the device using a sui- table carrier, even for a transport within a building, and separate it from the stand (see Section 5.2). CAUTION – Tilting danger! If the unit is tilted too much during lifting, risk of tipping exists.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Description of the device Overall view • Fig. 3: Plenum assembly [3] with plenum for downflow blower [19] and plenum for exhaust air blower [20]. The downflow filter and the exhaust air filter are in- stalled immediately to the pertaining plenum.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Description of the device Safety system The safety system comprises a combination of protective and alarm systems that ensure maximum personal and material protection. Safety systems: • Vacuum-sealed air system A vacuum-sealed air system in combination with HEPA filters for downflow and exhaust air forms the basis of the safety system for personal and ma- terial protection.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Description of the device • Position monitoring The position sensors detect the opening state of the front window and indicate whether the window is open or closed in the work position. • SmartFlow Indicatordisplay...
Operating Instructions Biological Safety Cabinet | MSC-Advantage Description of the device Operating and display elements Fig. 5: The biological safety cabinet is operated using an operating panel consisting of keys, function indicators and a display. [A] The display with its 5-digit indicator panel shows the following information, re- gardless of the activated operating function: –...
Operating Instructions Biological Safety Cabinet | MSC-Advantage Description of the device Sample chamber access Fig. 6: The manually movable front window [1] made of multi- layer security glass seals the safety sample chamber front side The sample chamber is accessible through various positions of the front window.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Description of the device Device interfaces Fig. 7: The standard equipment includes: • outlets for internal/external power supply, • bushings on both sides for cables, media valves, and hoses, • a communication port, •...
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Operating Instructions Biological Safety Cabinet | MSC-Advantage Description of the device CAUTION – Unstable underpressure! Do not switch the unit off as long as cables/ hoses are laid through the bushings. If breached bushings are not used in operati- on, new covers must be inserted in the ope- nings to ensure stable underpressure in the utility chamber (see Chap.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Description of the device Sample chamber illumination Fig. 8: The sample chamber illumination unit [2]: • one flourescent tube for model MSC 1.2, • two flourescent tubes for model MSC 0.9, 1.5,1.8, is installed behind the light dome [3].
Operating Instructions Biological Safety Cabinet | MSC-Advantage Start-up Initial operation Prior to the initial operation, the biological safety cabinet must be subjected to an installation test. Correct assembly and instal- lation performed by the operator are essential for good start-up.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Start-up Unlocking the transport protection Fig. 11: The front window counterweight [1] is secured to the device backpanel to protect it from transport damage. CAUTION – Unlocking the counterweight! The counterweight at the device backpanel compensates for the weight of the front win- dow.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Start-up Power supply connection WARNING – High voltage! Contact with current-carrying components may cause a lethal electric shock. Before connecting the device to the power sup- ply system, check plug and power supply cable for possible damage.
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Operating Instructions Biological Safety Cabinet | MSC-Advantage Start-up • The routine starts automatically when the front window is in the work posi- tion and if the air flows freely and steadily. If the airflow is disturbed during this run time, the process is cancelled and the calibration routine is restar- ted.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Start-up Installation test Do not operate the device before an initial operation with installation test has been performed after the final installation of the device. • The installation test must be performed in accordance with the specificati- ons of EN 12469 / 2000.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Handling and control Operating panel Fig. 12: The operating panel is the major display and operating element and has been divided into three functional units: • Display [1], keys [2] - [7], status indicators [8] - [18] 6.1.1...
Operating Instructions Biological Safety Cabinet | MSC-Advantage Handling and control Status indicators, Fig. 12: [8] The air system is operated at reduced capacity (the yellow LED is illuminated), [9] Airflow is not steady (the red LED is illuminated), [10]Airflow is steady (the green LED is illuminated), [11] Front window is not in work position (the red LED is illuminated), [12]Front window is in work position (the green LED is illuminated).
Operating Instructions Biological Safety Cabinet | MSC-Advantage Handling and control 6.1.4 Failure messages Failure messages are shown on the display as text/number combinations with the codes ER 3 to ER 7. If one of these codes appears on the display, contact Technical Service immediately.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Handling and control Description of the operating modes The following operating modes exist for the device: • Calibration mode (after initial start-up) • OFF mode • Window open mode • Work mode •...
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Operating Instructions Biological Safety Cabinet | MSC-Advantage Handling and control Standby mode: The front window can be lowered to seal the sample chamber. The air system output is reduced to match the lower air requirement. • The front window is closed: –...
Operating Instructions Biological Safety Cabinet | MSC-Advantage Operation Hygiene preparations for the sample chamber The sample chamber surfaces and the accessories required for the work process must be disinfected and cleaned in accordance with the hygiene guidelines set forth for the application.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Operation Work rules The observance of work rules ensures a minimum of operational safety when handling the biological safety cabinet. Before starting an operation: • Take off jewelry. • Put on required personal protective gear, e.g. hand, face, or body protection.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Shut-down Interrupting an operation To interrupt a work process: 1. Remove all samples from the biological safety cabinet and store them properly. 2. Remove accessories from the sample chamber and clean and disinfect them.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Cleaning and decontamination Decontamination procedure Several procedures can be applied for decontaminating the biological safety cabinet. Which procedure is selected, depends on: • the potential risk imminent in the agents, • the degree of purity required by an experiment or by a work process.
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Operating Instructions Biological Safety Cabinet | MSC-Advantage Cleaning and decontamination Predisinfection: 1. Remove all samples from the sample chamber and store them properly. 2. Remove accessories from the biological safety cabinet and disinfect them using the disinfection procedure recommended by the manufacturer.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Cleaning and decontamination UV disinfection after a wipe/spray disinfection A UV disinfection can be performed either by using the optional integral UV lamps or by using a mobile UV device. 9.3.1 UV disinfection using the integral UV lamp To start the UV disinfection routine: 1.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Cleaning and decontamination Disinfection with formaldehyde Procedure: For gas disinfection, formaldehyde is evaporated in the tightly sealed sample chamber. The quantity of the formaldehyde used depends on the sample chamber volume of the cabinet version to be disinfected (see Technical Data). Per cu- bic meter of sample chamber volume, at least 5 g formaldehyde must be evapo- rated with 20 ml water (corresponds with 25 ml of a 20 % formaldehyde solution).
Operating Instructions Biological Safety Cabinet | MSC-Advantage Cleaning and decontamination Cleaning the floorpan Clean the floorpan using a solution of tepid water and commer- cially available dishwashing agent. 1. Remove the workplates from the sample chamber. 2. Remove dirt residues and deposits thoroughly.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Maintenance 10.1 Inspection The SmartFlow Insicator, the test routine for the biological safety cabinet, deter- mines the cabinet's system status by way of adjusting various device parameters. • An inspection of the device should be performed if the SmartFlow Indicator displays steady 2 yellow LED`s.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Maintenance 10.2.1 Sample chamber illumination Fig. 18: The sample chamber is illuminated by one or two flu- orescent tubes (model MSC 0.9, 1.2, 1.5, 1.8). The spring-loa- ded sockets of the fluorescent tube [2] are installed to the light dome mounting frame in the sample chamber.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Maintenance 10.4 Exchanging SmartPorts NOTE – Contamination In order to prevent contaminated particles escaping from the utility chamber, the unit must be switched on in order to exchange the SmartPorts. The SmartPorts are removed inwards into the utility chamber.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Disposal 11.1 Disposal procedure Discarded cabinets or device components contain reusable materials. All com- ponents with the exception of the HEPA filters can be discarded after having been thoroughly cleaned and disinfected. The HEPA filters must be discarded in ac- cordance with the applicable national and state regulations for special solid waste.
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For further information on Thermo Scientific‘s compliance with these Directives, contact the recyclers in your country, and information on Thermo Scientific products wich may assist the detection of substances sub- ject to the RoHS Directive at www.thermo.com/WEEERoHS.
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Operating Instructions Biological Safety Cabinet | MSC-Advantage Technical data Volume, weights, and loads MSC 0.9 MSC 1.2 MSC 1.5 MSC 1.8 Description Volume Unit volume Floorpan Weights Cabinet Stand Loads Max. load per working area module Max. load on overall working...
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Operating Instructions Biological Safety Cabinet | MSC-Advantage Technical data Electrical data MSC-Advantage MSC 0.9 MSC 1.2 MSC 1.5 MSC 1.8 Voltage Rated voltage 1/N/PE AC, 230 V - 50-60 Hz Blower voltage 48 V / DC Current Power consumption max.
Operating Instructions Biological Safety Cabinet | MSC-Advantage Device log Device type: Part number: Serial number: Service number: Location: Operator's note: Work carried out Notes Date Signature...
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