Thermo Scientific HM 450 Instruction Manual
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Thermo Scientific HM 450 Instruction Manual

Thermo Scientific HM 450 Instruction Manual

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Thermo Scientific Sliding Microtome Microm HM 450
Thermo Scientific
Sliding Microtome
Microm HM 450
INSTRUCTION MANUAL
Microm International GmbH
1
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 760 - English

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  • Page 1 Thermo Scientific Sliding Microtome Microm HM 450 Thermo Scientific Sliding Microtome Microm HM 450 INSTRUCTION MANUAL Microm International GmbH part of Thermo Fisher Scientific Otto-Hahn-Str. 1A 69190 Walldorf / Germany 387 760 - English...
  • Page 2 Thermo Scientific Sliding Microtome Microm HM 450 Microm International GmbH part of Thermo Fisher Scientific Otto-Hahn-Str. 1A 69190 Walldorf / Germany 387 760 - English...
  • Page 3 This instruction manual will be supplied together with each instrument. Further copies can be ordered at the nearest Thermo Scientific sales office by giving the serial number of the instrument, the number of the instruction manual and the date of issue.
  • Page 4 Before operating the instrument, please read these instructions carefully to familiarize you with its proper operation and functions. The Thermo Scientific Sliding Microtome Microm HM 450 is a highly efficient sliding microtome for paraffin sectioning in routine. Only skilled or specially trained personnel must operate the microtome, i.e.
  • Page 5 Thermo Scientific Sliding Microtome Microm HM 450 Intended use Table of contents Safety Precautions Part 1 Introduction Description Sliding Microtome HM 450 Technical Data HM 450 Part 2 Operating Instructions Setting up the microtome Initial turn-on Cutting movement and retraction...

  • Page 6: Table Of Contents

    Thermo Scientific Sliding Microtome Microm HM 450 2-12 Standard equipment 2-13 Additional equipment (optional) 2-13-1 Disposable blade holder 2-13-2 Specimen clamps 2-13-3 Height adjustment plates 2-13-4 Microtome knives 2-13-5 Additional accessories Part 3 Theory of operation Cutting movement Specimen coarse feed and trimming function...
  • Page 7 Thermo Scientific Sliding Microtome Microm HM 450 SAFETY PRECAUTIONS WARNING SIGNALS AND SYMBOLS The installation and routine use of the Sliding Microtome HM 450 is easy and safe if the instructions in this manual are being observed. Note: Special instructions regarding operation of the instrument.
  • Page 8 Please observe the following general precautions during operation of this instrument. Failure to comply with these precautions violates safety standards and the intended use of the instrument. Thermo Scientific is not liable for misuse of the instruments and failure to comply with basic safety requirements.
  • Page 9 Thermo Scientific Sliding Microtome Microm HM 450 HAZARD OF INFECTION Specimens used during the intended operation of the instrument might potentially be infectious. For this reason, it is recommended to observe the general laboratory regulations concerning protection against danger of infection.
  • Page 10 Thermo Scientific Sliding Microtome Microm HM 450 Fig. 1 Microm International GmbH part of Thermo Fisher Scientific Otto-Hahn-Str. 1A 69190 Walldorf / Germany 387 760 - English...
  • Page 11 The cross roller bearings on sledge and block allow non-tiring working and a smooth sliding movement with optimal stability. The HM 450 will cut sections from 0 µm to 100 µm. The specimen clamping can be moved up and downwards via the two arrow-shaped coarse feed buttons on the left side of the instrument. This way, specimen and knife edge distance can be adjusted quickly.
  • Page 12 Thermo Scientific Sliding Microtome Microm HM 450 TECHNICAL DATA HM 450 Microtome: Section thickness range: ..........0,5 - 100 µm Resolution: ..........0,5 µm for 0,5...3 µm ............... 1 µm for 3...10 µm ..............2 µm for 10...20 µm ..............5 µm for 20...60 µm ..............
  • Page 13 Thermo Scientific Sliding Microtome Microm HM 450 PART 2 OPERATING INSTRUCTIONS 2-1 SETTING UP THE MICROTOME • Unpack the microtome and remove the wrapping. • To carry the instrument, lift it on the front and rear side from the lower side.
  • Page 14 Thermo Scientific Sliding Microtome Microm HM 450 INITIAL TURN-ON Note: The kind of the used examination Microm GmbH materials and all special conditions for part of Thermo Fisher Scientific their processing, pre-treatment and, if 69190 Walldorf, Germany Type HM 450 necessary, storage as well as instrument controls Ser.
  • Page 15 Thermo Scientific Sliding Microtome Microm HM 450 CUTTING MOVEMENT AND RETRACTION • To start the cutting movement of the microtome, move the sledge horizontally. • The knife (or blade) which is clamped into the knife carrier is drawn horizontally over the specimen towards the user.
  • Page 16 Thermo Scientific Sliding Microtome Microm HM 450 LOCKING THE SLEDGE For the protection against injury by unintended movements of the knife sledge, the microtome is equipped with a lock of the sledge in any position. Caution: The sledge should be locked for the user's personal safety, e.g.
  • Page 17 Thermo Scientific Sliding Microtome Microm HM 450 2-4-1 ADDITIONAL BALLAST FOR SLEDGE As required by users to vary the weight of the sledge, we have included additional ballast (Fig. 4- Additional Ballast 3-1) into the standard equipment. To assemble the additional ballast, please proceed as described below: •...
  • Page 18 Thermo Scientific Sliding Microtome Microm HM 450 SETTING SECTION THICKNESS AND TRIMMING THICKNESS The required section thickness and trimming thickness is set via the operating knob on the left side of the instrument. To choose between section thickness and trimming thickness, press the operating knob (fig.
  • Page 19 Thermo Scientific Sliding Microtome Microm HM 450 SPECIMEN FEED 2-6-1 COARSE FEED AND SPEED FOR COARSE FEED For the fast up and downward travel between specimen and knife edge, the microtome has a coarse feed with vertical direction. • The specimen clamping can be moved 40 mm vertically up or downwards via the two coarse feed buttons (fig.
  • Page 20 Thermo Scientific Sliding Microtome Microm HM 450 2-6-2 TRIMMING AND FIRST CUTS After the specimen and the knife are adjusted, further gradual feed for trimming can be carried out using function "trimming". different sectioning series, deeper layers of the specimen can be reached with the function "trimming".
  • Page 21 Thermo Scientific Sliding Microtome Microm HM 450 2-6-3 FINE FEED, MANUAL After having adjusted knife and specimen as well as having trimmed the specimen, sectioning can be started. The fine feed can be carried out either manually or automatically. The desired feed mode can be selected via the submenu "Automatic/Manual".
  • Page 22 Thermo Scientific Sliding Microtome Microm HM 450 2-6-5 RETRACT FUNCTION FOR COMPENSATING THE THERMAL EXTENSION Normally the ambient air slowly warms up the paraffin specimen which was cooled down before. Due to the rising temperature, the paraffin expands and the achieved sections will be slightly thicker than the pre-selected value.
  • Page 23 Thermo Scientific Sliding Microtome Microm HM 450 OPERATING PANEL The ergonomically shaped operating panel has a large, illuminated graphical display (fig. 7.1) showing clearly the respective operating states and settings. The two arrow-shaped LEDs (fig. 7.2) blink, when the specimen is just in its upper or lower end position.
  • Page 24 Thermo Scientific Sliding Microtome Microm HM 450 KEYBOARD Below-mentioned the four buttons of the operating panel and their meaning signification on the operating panel are described. 2-8-1 SCROLL BUTTON Via this button (fig. 8) different information (fig. 9) can be called in the second line on the display: •...
  • Page 25 Thermo Scientific Sliding Microtome Microm HM 450 2-8-3 MEMORY BUTTON The memory function (fig. 11) is used to rapidly find again a stored first cut position. Pre-condition MEMO for this is that blocks are cut which have been embedded in the same mold and always have approximately the same height.
  • Page 26 Thermo Scientific Sliding Microtome Microm HM 450 2-8-4 MENU BUTTON Via the menu button (fig. 12) you have access to further settings which are described below. • Normally these settings are made when MENU starting to work and do not have to be altered while sectioning.
  • Page 27 Thermo Scientific Sliding Microtome Microm HM 450 2-8-4-2 RETRACTION ON/OFF Retraction R:ON The instrument is equipped with an electronic retraction which can be turned off. During the return travel of the knife, the specimen is drawn back (away from the knife). This way, the knife does not touch the specimen surface during Fig.
  • Page 28 Thermo Scientific Sliding Microtome Microm HM 450 2-8-4-3 SPEED FOR COARSE FEED In this menu, the speed for the coarse feed can be selected in three different settings. Speed äää • Turn the selection knob to alter the number of Fig.
  • Page 29 Thermo Scientific Sliding Microtome Microm HM 450 2-8-4-5 SELECTING THE DISPLAY MODE Here the display mode NORMAL and BIG can be selected (fig. 20). Display mode NORMAL • The normal display mode (fig. 21) shows the selected feed and trim thicknesses with additional status indications.
  • Page 30 Thermo Scientific Sliding Microtome Microm HM 450 2-8-4-6 TIME AND DATE With this menu the current time as well as the current date (fig. 23) can be set. Time & Date • Press the menu button. The submenu is shown 14:26 28.10.02...
  • Page 31 Thermo Scientific Sliding Microtome Microm HM 450 SPECIMEN CLAMPING To clamp specimens, different specimen clamping systems are available. With the orienting adapter it is simple to align the specimen properly in relation to the knife. 2-9-1 STANDARD SPECIMEN CLAMP (Cat. no. 715480) The standard specimen clamp is used for rectangular and square paraffin blocks.
  • Page 32 Thermo Scientific Sliding Microtome Microm HM 450 2-9-3 UNIVERSAL CASSETTE CLAMP (Cat. no. 715500) The universal cassette clamp represents a quick change system standardized embedding cassettes. • To insert or remove cassettes, press the lever (fig. 25.1) upwards. Warning: When inserting the universal cassette Fig.
  • Page 33 Thermo Scientific Sliding Microtome Microm HM 450 2-10 CHANGING SPECIMEN CLAMPS Due to the quick change system of the specimen clampings, specimen clamps can be easily and rapidly changed without needing any tools. • Loosen the clamping of the specimen clamp (fig.
  • Page 34 Thermo Scientific Sliding Microtome Microm HM 450 2-11 KNIFE CARRIER Hazard of hand injuries: Due to moving parts in connection also with the microtome knife, a danger area arises, which might lead to hand injuries in case of non-compliance with the safety features of the microtome and when disregarding the instruction manual.
  • Page 35 Thermo Scientific Sliding Microtome Microm HM 450 Protection against injury • The knife carrier is equipped with a knife guard (fig. 27.4) which should be used while knife or specimen are adjusted. Caution: Please note that knives with a length of more than 16 cm (e.g.
  • Page 36 Thermo Scientific Sliding Microtome Microm HM 450 2-11-1 BLADE HOLDER FOR DISPOSABLE BLADES Two different types of disposable blades are available: low profile blades (height: 8 mm) and Clearance angle adjustment high profile blades (height: 14 mm). When using •...

  • Page 37: Standard Equipment

    Thermo Scientific Sliding Microtome Microm HM 450 2-12 STANDARD EQUIPMENT The sliding microtome HM 450 is supplied with the following accessories: 1 hex head wrench 5 mm 1 hex head wrench 6 mm 1 cover 4 rubber feet with lower height...

  • Page 38: Height Adjustment Plates

    Thermo Scientific Sliding Microtome Microm HM 450 2-13-3 HEIGHT ADJUSTMENT PLATES 5 mm 448070 10 mm 440400 20 mm 440410 2-13-4 MICROTOME KNIVES Steel knives, type c 12 cm 152010 16 cm 152020 18,5 cm 152270 22 cm 152030 25 cm...

  • Page 39: Part 3 Theory Of Operation

    PART 3 THEORY OF OPERATIONG CUTTING MOVEMENT SPECIMEN CLAMPING SYSTEM AND SPECIMEN ORIENTATION The electronic sliding microtome HM 450 has a high-precise, zero-backlash linear roller bearing According to the form and size of the specimens, (cross roller bearing) for the bearing of the knife...

  • Page 40: Part 4 Working With The Microtome

    Thermo Scientific Sliding Microtome Microm HM 450 PART 4 WORKING WITH THE MICROTOME PREPARATION AND ORIENTATION SECTIONING INSTRUCTIONS Hazard of hand injuries: Before sectioning, the specimens must be prepared appropriately and embedded in suitable Due to moving parts in connection also media.

  • Page 41: How To Avoid Malfunctions

    Thermo Scientific Sliding Microtome Microm HM 450 HOW TO AVOID MALFUNCTIONS 4-4-5 ADJUSTMENT OF THE KNIFE Note: Take care to adjust the proper clearance angle of In case of malfunctions and/or service the knife. work, please turn off the instrument and contact your local dealer.

  • Page 42: Part 5 Maintenance

    Thermo Scientific Sliding Microtome Microm HM 450 PART 5 MAINTENANCE Annual routine maintenance To secure section quality and functioning of the microtome, it is recommended that a routine maintenance be performed by a trained service technician once a year. Service contract...

  • Page 43: Part 6 Cleaning And Care

    Thermo Scientific Sliding Microtome Microm HM 450 PART 6 CLEANING AND CARE Cleaning intervals Cleaning, care and decontamination of the microtome depends frequently microtome is used. For optimal sectioning, the instrument must be free of section waste, especially the guides and clamping mechanism.

  • Page 44: Part 7 Conditions For The Transportation Of The Instrument

    NOT to below-mentioned measures repair. To order original packaging materials, transportation preparation. please contact Thermo Scientific l or your local, by • In addition, the conditions for storage and Thermo Scientific authorized, dealer. transportation as mentioned in part 1-2 must be observed during the entire transportation.
  • Page 45 Thermo Scientific Sliding Microtome Microm HM 450 For transportation outside closed buildings, please observe following additional measures: • Turn off the instrument. • Unplug the unit. • Remove knife or blade and store it in a safe way. • Secure the sledge via the transportation safety sheet: Place the sheet onto the specimen holder.
  • Page 46 Thermo Scientific Sliding Microtome Microm HM 450 DISPOSAL OF THE INSTRUMENT AFTER FINAL SHUTDOWN After the final shutdown of the instrument, we recommend to contact a local recycling company for the disposal according to the national applicable regulations. To be applied in the countries of...

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