Millipore Spectroquant Multy Operating Manual page 60

Measuring range: 0.5 – 3000
1)
after corresponding dilution (details see package insert)
Preparation and incubation:
Check the pH of the Fill 2 oxygen reaction
sample, specified range: bottles each with
pH 6 – 8. pretreated sample
If required, add dilute and 2 glass beads to
sodium hydroxide overflowing. Close
solution or sulfuric acid bubble-free with the
drop by drop to adjust slanted ground-glass
the pH. stoppers.
Determination:
Measurement of final
oxygen concentration
= Result 2
(measurement sample)
= Result 2
(blank)
After incubation, use Select method
one bottle of pretreated
sample and one of
inoculated nutrient-
salt solution for the
measurement of the final
oxygen concentration.
Transfer each solution Fill approx. 10 ml of
into a separate 16-mm distilled water into a
cell, close with the screw 16-mm cell (do not add
caps. any reagents!), close
with the screw cap.
(Blank cell)
Calculation:
BOD of measurement sample:
Result 1 – Result 2 (measurement sample) = A in mg/l
BOD of blank:
Result 1 – Result 2 (blank) = B in mg/l
BOD of original sample in mg/l = A • dilution factor – B
Release 08/2014 - Spectroquant
BOD
Biochemical Oxygen Demand
1)
mg/l O 16-mm cell
2
Fill 2 oxygen reaction
bottles each with inocu-
lated nutrient-salt solu-
tion and 2 glass beads
to overflowing. Close
bubble-free with the
slanted ground-glass
stoppers.
7 0 . Add to each oxygen
reaction bottle 5 drops of
BOD-1K and then 10
drops of BOD-2K, close
bubble-free, and mix for
approx. 10 seconds.
Insert the blank cell into
the cell compartment.
Press Zero .
®
Multy
Measurement of inital
oxygen concentration
= Result 1
(measurement sample)
= Result 1
(blank)
Use one bottle of
pretreated sample
and one of inoculated
nutrient-salt solution
for the measurement
of the initial oxygen
concentration.
Reaction time:
1 minute
Insert the blank cell
(nutrient-salt solution)
into the cell compart-
ment. Align the mark on
the cell with that on the
photometer.
Press Test .
Quality assurance:
To check the measurement system (test reagents, meas-
urement device, and handling) Spectroquant BOD Stan-
dard (analogous to EN 1899), Cat.No. 100718, can be
used.
100687
Cell Test
Incubate one bottle
of pretreated sample
and one of inoculated
nutrient-salt solution
closed in a thermostatic
incubation cabinet at
20 + 1°C for 5 days.
Add to each oxygen re-
action bottle 10 drops
of BOD-3K, reclose,
and mix.
Insert the cell containing
the sample into the cell
compartment. Align the
mark on the cell with
that on the photometer.
Press Test .