Millipore Spectroquant Multy Operating Manual page 166

Measuring range: 0.01 – 2.50 mg/l PO
0.03 – 7.66 mg/l PO
0.02 – 5.73 mg/l P
Attention: In contrast to the instructions given in the package insert the sample volume as well as the reagent volume
must be doubled.
Check the pH of the Select method 3 8 3 .
sample, specified range:
pH 0 – 10.
If required, add dilute
sulfuric acid drop by
drop to adjust the pH.
Add 2 level blue micro- Shake the cell vigor-
spoons of PO -2, close ously to dissolve the
4
with the screw cap. solid substance.
Important:
For the determination of total phosphorus = sum of
orthophosphate, polyphosphate, and organophos-
phate a pretreatment with Crack Set 10C, Cat.No.
114688, or Crack Set 10, Cat.No. 114687, and thermore-
actor is necessary.
Release 08/2014 - Spectroquant
Phosphate
Determination of orthophosphate
-P 24-mm cell
4
24-mm cell
4
O 24-mm cell
2 5
Fill approx. 10 ml of
distilled water into a
24-mm cell (do not add
any reagents!), close
with the screw cap.
(Blank cell)
Reaction time:
5 minutes
Press Enter to start
the countdown.
®
Multy
Pipette 10 ml of the
sample into a 24-mm
cell.
Insert the blank cell into
the cell compartment.
Align the mark on the
cell with that on the pho-
tometer. Press Zero .
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) we recommended
®
to use Spectroquant CombiCheck 10, Cat.No. 114676.
Use 10 ml R-1 instead of the sample.
Ready-for-use phosphate standard solution Certipur
Cat.No. 119898, concentration 1000 mg/l PO
be used after diluting accordingly.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 10) is highly recommended.
Use 10 ml sample + 0.1 ml R-2.
114848
Test
Add 10 drops of PO -1,
4
close with the screw
cap, and mix.
Insert the cell containing
the sample into the cell
compartment. Align the
mark on the cell with
that on the photometer.
Press Test .
®
,
3–
, can also
4