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Table of Contents
Related Manuals for Millipore Milliflex Oasis
Summary of Contents for Millipore Milliflex Oasis
- Page 1 User Guide Milliflex Oasis system ® Important: For an animated interactive user guide, visit SigmaAldrich.com/Milliflex-Oasis for a visual understanding of the different operations. The life science business of Merck operates as MilliporeSigma in the US and Canada. SigmaAldrich.com...
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Page 2: Table Of Contents
Table of Contents Introduction 5. Performance verification 1. Components 6. Use of multiple pumps Required items Material needed 6.1.1 Two pumps connected Description of items 6.1.2 Three pumps connected Touch switch Installation 2. Hardware installation 7. Culture media growth promotion test 3. -
Page 3: Introduction
The Milliflex Oasis system for microbiological examination of aqueous fluids is composed ® of the Milliflex Oasis pump, the Milliflex Oasis single-use filtration unit and the Milliflex ®... -
Page 4: Components
® for each additional pump. Consumables: please refer to the ordering information section for details on the different items. 1. Filtration units 2. Culture media 3. Consumables for the Milliflex Oasis internal flow sanitization kit ® Page 4 of 32... -
Page 5: Description Of Items
2. Two filtration heads with their covers plug and gasket removal tool 3. Tubing for discarding the liquids 2. Vacuum gauge 4. Accessory kit 5. Spare parts kit Figure 3: Milliflex Oasis internal flow sanitization kit ® (MMSANKIT1) package components Figure 1: Milliflex Oasis pump (MMSYSTMM1) ®... -
Page 6: Touch Switch
The switch is touch-sensitive. Use your fingertip to activate it. Touch the center of the switch without touching the outer ring. When the Milliflex Oasis pump is on ® standby mode, it can be reactivated by touching the space between the two switches. -
Page 7: Hardware Installation
• Place the pump in the working area When using the Milliflex Oasis ® system under a horizontal laminar flow hood (LFH), the front of the pump should be placed at least at 160 mm (6.3 in.) from the front... - Page 8 ® cable and tubing (item MMTUGUIDE). Figure 12: colored ring installation – part 2 Figure 10: use of Milliflex Oasis guides for ® electrical cable and tubing (item MMTUGUIDE) • Clean the pump heads using a wipe moistened with a surface sanitizer listed •...
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Page 9: Testing
Optional: if you need four filtration units before starting your sample processing: or less for your testing, open the bag at the front of the safety cabinet, then pull • Milliflex Oasis pump installed following ® out only half of the tray, separate the tray the installation section in two and place the “half-tray”... -
Page 10: Media Plates
• Open the filtration unit lid traceability information and a 2D code, are present on each bag. It is possible to • Pour the sample into the Milliflex Oasis ® detach the stickers and stick them into a filtration unit and flip down the lid without log book. - Page 11 • When the dry-out cycle is finished, the • Open the pre-opened media plate with pump stops, and the switch light stops one hand blinking; firmly press the filtration unit lid Note: Be careful not to touch the agar down to lock it while removing the media plate cover.
- Page 12 • Open the filtration unit lid while Optional: empty filtration unit trays can be maintaining the base with the other hand used to transport and to incubate the media plates/membrane assembly. When placing • With the same hand, now pinch the media plates/membrane assemblies on the funnel to separate it from the membrane trays, the lid tab must be positioned out of...
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Page 13: Waste
Optional: it is possible to use an accessory Note: If some filtered liquid remains in the pump head, it is possible to remove to remove the support, the Milliflex Oasis ® membrane support removal tool (item it with a dry, sterile wipe. Regardless of MMSUPREMV). -
Page 14: Cleaning & Maintenance
4. Cleaning & Maintenance 4.1 Cleaning & Maintenance table First Before each After each Component Action installation testing shift testing shift Monthly Yearly Filtration head Cleaning Pump Surface cleaning Pump Internal flow sanitization Pump Rinsing If testing non- water samples Filtration head External gasket If testing non-... -
Page 15: Filtration Head Cleaning
If samples other than water have been processed, filter 300 mL of purified water at the end of each day. This is recommended to ensure the functionality of internal parts. Use either a Milliflex Oasis filtration unit ® or the internal flow sanitization kit with a... -
Page 16: Pump Internal Flow Sanitization
4.7 Pump internal flow • Take one sanitization funnel assembly (sanitization kit consumable) and remove sanitization its support, then place the funnel on the Materials: • Take a syringe (sanitization kit 1. Sanitization kit (item MMSANKIT1) consumable) and remove its plunger 2. -
Page 17: Filtration Head Complete Cleaning
4.8 Filtration head Note: the switch starts blinking for 15 minutes while the following operations complete cleaning occur: Materials: 1. The plunger is partially pulled down and the sanitizer fills the pump internal flow 1. Pump head path. 2. Gasket removal tool (to be found in the 2. - Page 18 • Remove the black check valve with the • Put the gaskets and check valve back in metal tool provided in the gasket kit place using the gasket removal tool Figure 38: check valve removal with tool — part 1 Figure 40: check valve installation with tool —...
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Page 19: Replacement
4.9 Filtration head 4.10 Sanitization kit gasket and check gaskets (spare parts) valve (spare parts) replacement replacement Materials: Materials: 1. Sanitization kit (item MMSANKIT1) 1. Pump head 2. Sanitization kit gasket kit (item MMGASKTSK) containing: 2. Spare part kit to be found in the pump package or filtration head gasket kit •... -
Page 20: Performance Verification
10. Repeat steps 4 to 9 twice more (sanitization kit consumables) on the sanitization kit Check that the average values are within the • Connect the Milliflex Oasis vacuum gauge ® following specifications. to the syringe port of the kit... -
Page 21: Use Of Multiple Pumps
6. Use of multiple pumps Up to three pumps can be connected 6.1.2 Three pumps connected: providing the following benefits: • Three Milliflex Oasis ® pumps (item • Higher throughput MMSYSTMM1) • Use of single power supply • Two Milliflex Oasis ®... - Page 22 LFH (see picture). until a click is heard • Plug the DC power cord in the free power port, at the back of the Milliflex Oasis ® pump A • Connect the free power cord to the power supply •...
- Page 23 • For each pump, remove the two pump heads from their package and remove the yellow covers Optional: From the accessory bags, select colored rings, clean them using a wipe and one of the sanitizers recommended in the cleaning & maintenance section, then install them on the pump heads.
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Page 24: Culture Media Growth Promotion Test
• Place the media plates in the working area • Incubate the media plate assembly at the specified temperature and time • Place a tray of Milliflex Oasis funnels ® without membranes (item MMRECVERY) in Note: Incubate the media plate assembly the working area with the lid facing down. -
Page 25: Cassettes
® These products (older generation) can be Milliflex empty cassettes ® used instead of Milliflex Oasis ® media plates. Empty cassettes are available for those These products do NOT have the following users who would like to prepare their own features: culture media. - Page 26 Milliflex empty cassette for liquid • Squeeze the ampoule to dispense the ® media (item MXLMC0120) medium into the cassette. Use the entire content of the ampule. Use the following steps to prepare a liquid Note: Avoid pressing the cassette on medium cassette: the working surface while inserting the •...
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Page 27: System Specifications
9. System specifications Milliflex Oasis pump Milliflex Oasis media plates ® ® Frame Acrylonitrile Styrene Acrylate and Material Polystyrene (PS) Polycarbonate, compatible with R2A plate color Blue standard UV sterilization TSA plate color Green Filtration heads Two per pump, Stainless steel,... -
Page 28: Ordering Information
10. Ordering information Description Qty/pack Article number Filtration units Milliflex Oasis 100 mL funnel, 0.45 µm white gridded, mixed cellulose esters (MCE) membrane MMHAWG124 ® Milliflex Oasis 250 mL funnel, 0.45 µm white gridded, mixed cellulose esters (MCE) membrane MMHAWG224 ®... - Page 29 Milliflex Oasis guides for electrical cable and tubing MMTUGUIDE ® Milliflex Oasis membrane support removal tool MMSUPREMV ® Stickers and filtration head rings for customizing the Milliflex Oasis pump MMCUSTKIT ® Milliflex Quantum membrane transfer tool for Milliflex Oasis system MMHEADQU1 ®...
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Page 30: Troubleshooting
11. Troubleshooting Issue Solution Condensation on media plates The media plates must be at ambient temperature for optimal growth performance. If plates are stored in a fridge, warming it up to 2 hours is recomended The pump switch is not active Check that there is no liquid on the switch Small amount of liquid in the pump head after filtration It is normal to observe a small amount of liquid in the... -
Page 31: Operator & Equipment Safety Instructions
Remaining liquid in the funnel or in the syringe The test was uncomplete, e.g. due to a power issue during the process. Restart the sanitization from the start 12. Operator & equipment safety instructions Please refer to the Operator & Equipment instructions addendum SigmaAldrich.com/Milliflex-Oasis 13. - Page 32 For Technical Service visit: SigmaAldrich.com/techservice SigmaAldrich.com © 2020 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved. Merck, the vibrant M, Milliflex, Milliflex Oasis, Millipore VM_UG3243EN Ver. 1.0 and Sigma-Aldrich are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates. All other trademarks are the property of their 18737 respective owners.
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