Millipore 8003 User Manual
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Millipore 8003 User Manual

Stirred ultrafiltration cells
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Stirred Ultrafiltration Cells
Models 8003, 8010, 8050, 8200, 8400
User Guide

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Summary of Contents for Millipore 8003

  • Page 1 Stirred Ultrafiltration Cells Models 8003, 8010, 8050, 8200, 8400 User Guide...
  • Page 2 This manual is believed to be complete and accurate at the time of publication. In no event shall Millipore Corporation or an affiliated corporation be liable for incidental or conse- quential damages in connection with or arising from the use of this manual.

  • Page 3: Table Of Contents

    Contents Introduction ..........1 Models Available ........1 Safety Features ........1 Model 8003 Parts List ......2 Model 8010 Parts List ......4 Model 8050 Parts List ......6 Model 8200 Parts List ......8 Model 8400 Parts List ......10 Operating Modes ........
  • Page 4 Sterilization ........... 21 Storage ..........22 Maintenance ......... 22 Troubleshooting ........23 Specifications ........24 Materials of Construction ...... 24 Compliance with the Pressure Equipment Directive, 97/23/EC ....... 25 Product Ordering Information....26 Technical Assistance......30 Standard Warranty ........ 30...

  • Page 5: Introduction

    Introduction Millipore stirred ultrafiltration cells are designed for rapid concentration or purification of macromolecular solutions in volumes from 3 to 400 mL. All models are used in conjunction with a stirring table. The table is magnetically coupled to a stirring bar, which maintains fluid movement during operation, thereby reducing the negative effects of concentration polarization (i.e., the...

  • Page 6: Model 8003 Parts List

    Model 8003 Parts List � � � � � � � � � � � � � �...
  • Page 7 Stirred Ultrafiltration Cells Model 8003 Parts List, continued Item Description Quantity Supplied Cap Assembly Pressure Relief Valve — O-Ring Stirrer Assembly Body O-Ring Membrane — Membrane Holder Elastomeric Tubing 4 in. Base Tube Fitting Assembly Tubing, Plastic 4 ft Stand Assembly...

  • Page 8: Model 8010 Parts List

    Model 8010 Parts List � � � � � � � � � � � � �...
  • Page 9 Stirred Ultrafiltration Cells Model 8010 Parts List, continued Item Description Quantity Supplied Cap Assembly Pressure Relief Valve — O-Ring Stirrer Assembly Body O-Ring Membrane — Membrane Holder Elastomeric Tubing 4 in. Base Tube Fitting Assembly Tubing, Plastic 4 ft Stand Assembly...

  • Page 10: Model 8050 Parts List

    Model 8050 Parts List � � � � � � � � � � � � �...
  • Page 11 Stirred Ultrafiltration Cells Model 8050 Parts List, continued Item Description Quantity Supplied Cap Assembly Pressure Relief Valve — O-Ring Stirrer Assembly Body O-Ring Membrane — Membrane Holder Elastomeric Tubing 4 in. Base Tube Fitting Assembly Tubing, Plastic 4 ft Stand Assembly...

  • Page 12: Model 8200 Parts List

    Model 8200 Parts List � � � � � � � � � � � � �...
  • Page 13 Stirred Ultrafiltration Cells Model 8200 Parts List, continued Item Description Quantity Supplied Cap Assembly Pressure Relief Valve — O-Ring Stirrer Assembly Body O-Ring Membrane — Membrane Holder Elastomeric Tubing 4 in. Base Tube Fitting Assembly Tubing, Plastic 4 ft Stand Assembly...

  • Page 14: Model 8400 Parts List

    Model 8400 Parts List � � � � � � � � � � � � �...
  • Page 15 Stirred Ultrafiltration Cells Model 8400 Parts List, continued Item Description Quantity Supplied Cap Assembly Pressure Relief Valve — O-Ring Stirrer Assembly Body O-Ring Membrane — Membrane Holder Elastomeric Tubing 4 in. Base Tube Fitting Assembly Tubing, Plastic 4 ft Stand Assembly...

  • Page 16: Operating Modes

    Diafiltration Connect the ultrafiltration cell to an auxiliary reservoir (Millipore cat. no. 6028) containing a diafiltration solution with the desired microsolute concentration. The use of stirred cells in the diafiltration mode also requires use of the CDS-10 Concentration/Dialysis Selector (Milipore cat.

  • Page 17: Unpacking The System

    Stirred Ultrafiltration Cells Unpacking the System Place cell on a level surface and remove packing inserts. Turn the pressure relief valve knob to the hori- zontal position. Hold the body and push the cap down. Slide the cell out of the retaining stand and remove the cap assembly with a twisting motion.

  • Page 18: Setting Up The System

    Model 8003 only: Invert the cell body and place the smaller membrane holder O-ring (Model 8003 Parts List — N) into the recess. Gently tap to ensure that the O-ring remains when the body is upright. Place the membrane into the holder, shiny side up.
  • Page 19 Stirred Ultrafiltration Cells Stirrer Assembly Installation Place the stirrer assembly into the cell body. When properly installed, the arms of the stirrer assembly will rest on the small ridge inside the top of the cell body. Pour the sample into the cell. Push the cap assembly down onto the cell body, using a twisting motion and orienting the gas inlet port on the cap opposite the filtrate exit port on the...
  • Page 20 Gas Pressure Line Installation Attach the gas pressure line to the tube fitting on the cap as follows: Unscrew the hexagonal nut (a) from the cap assem- bly and remove the O-ring (d). ½” Slide the hex nut onto the end of gas tubing.

  • Page 21: Operating Guidelines

    Stirred Ultrafiltration Cells Operating Guidelines See membrane instructions for cleaning and storage ■ procedures, and for buffer and solvent compat- ibility. Highly viscous solutions filter slowly, as do solutions ■ containing particulate matter, such as colloids. Where a viscous agent (sucrose, glycerin, etc.) is to be removed, flow can often be increased by pre-dilution.

  • Page 22: Limitations

    Limitations This section explains the limitations of the stirred ultra- filtration cell. Pressure Limits Do not exceed 75 psi (5.3 kg/cm ) nitrogen gas pressure. CAUTION: Never operate the cell without its retaining stand. The cap will pop off when the cell is pressurized, and the liquid in the cell may spill or splatter.

  • Page 23: Cell Operation

    Stirred Ultrafiltration Cells Chemical Resistance, continued For other solvent compatibilities, consult a standard text or contact Millipore Technical Service. For the chemical resistance of disc membranes, see the instructions packed with the product. Cell Operation Select the cell operating mode as follows: For concentration: Connect the inlet line to a regulated gas pressure source.
  • Page 24 Cell Operation, continued When operating with hazardous ▲WARNING: or especially valuable materials, pressure-check the cell thoroughly, to ensure that all components have been properly assembled, before turning on the stirring table. Place the cell on the magnetic stirring table.

  • Page 25: Shutting Down The System

    Disassemble the cell, wash all components with a mild detergent/water solution and rinse thoroughly. Sterilization Millipore stirred ultrafiltration cells can be autoclaved at 121 °C (250 °F) for 30 minutes. They are compatible with standard sterilizing gas mixtures, 70% ethanol, isopropanol and 5% formalin.

  • Page 26: Storage

    Storage Disassemble the ultrafiltration cell whenever it is unlikely to be used for several weeks. Maintenance Replace O-rings at the first sign of damage or wear. ■ If installation or removal of the cap assembly ■ becomes difficult, lubricate the cap O-ring with a small amount of petroleum jelly or water.

  • Page 27: Troubleshooting

    Stirred Ultrafiltration Cells Troubleshooting Symptom: Little or No Filtrate Obtained Rotate the pressure-relief valve knob to check for ■ pressure. If the cell is not pressurized, check the nitrogen source and regulator. Make sure the glossy side of the disc membrane faces ■...

  • Page 28: Specifications

    Specifications Model No. 8003 8010 8050 8200 8400 Cell capacity (mL) Minimum process 0.075 10.0 volume (mL) Membrane diam. (mm) 44.5 63.5 Effective membrane 13.4 28.7 41.8 area (cm Hold-up volume (mL) 0.07 Empty weight, approx. (g) Retaining stand base 6 ×...

  • Page 29: Compliance With The Pressure Equipment Directive, 97/23/Ec

    The product is accompanied by user instructions and bears markings to permit identification of Millipore Corporation as the manufacturer or authorized representative of this product within the European Community.

  • Page 30: Product Ordering Information

    Product Ordering Information This section lists the catalogue numbers for the Model 8000 series Stirred Ultrafiltration Cells, accessories, and replacement parts. See Technical Assistance section for information about contacting Millipore. To order Millipore products on-line, go to www.millipore.com/products. Catalogue Description...
  • Page 31 Stirred Ultrafiltration Cells Product Ordering Information, continued...
  • Page 32 Catalogue Item* Description Qty/pk Number 8000SCPKIT 8000 Series Stirred Cell Replacement Part Kit (contains the following parts for Models 8003, 8010, 8050, 8200, 8400) Cap O-Rings Models 8003, 8010 Model 8050 Model 8200 Model 8400 Membrane holder O-Rings Models 8003, 8010...
  • Page 33 Stirred Ultrafiltration Cells Product Ordering Information, continued Catalogue Item* Description Qty/pk Number 8003SCPKIT Model 8003 Replacement Parts Kit (contains the following components) A, B Cap Assembly Stirrer Assembly Base 8010SCPKIT Model 8010 Replacement Parts Kit (contains the following components) A, B...

  • Page 34: Technical Assistance

    In the event of a breach of the foregoing warranty, Millipore’s sole obligation shall be to repair or replace, at its option, the applicable product or part thereof, provided the customer notifies Millipore promptly of any such breach.

This manual is also suitable for:

8010805082008400

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