Millipore Spectroquant Multy Operating Manual page 214

Standard solution of hydrogenperoxide
Preparation of a stock solution:
Place 10.0 ml of Perhydrol
formity-checked 100-ml volumetric flask and make up to the
mark with distilled water. Transfer 30.0 ml (full pipette) of this
solution to a calibrated or conformity-checked 1000-ml
volumetric flask and make up to the mark with distilled water.
The stock solution prepared according to this proce dure has a
concentration of approx. 1000 mg/l hydrogenperoxide.
Precise assay of the stock solution:
Pipette 50.0 ml (full pipette) of the hydrogenper oxide stock
solution into a 500-ml conical flask, dilute with 200 ml of
distilled water, and add 30 ml of sulfuric acid 25 %.
Titrate with a 0.02 mol/l potassium permanganate solution
until the color changes to pink.
Calculation and preparation of the standard solution:
Consumption of potassium permanganate solution 0.02 mol/l
(ml) x 34.02 = content of hydrogen peroxide, in mg/l
Further investigational concentrations may be prepared from
the stock solution exactly deter mined according to the pro-
cedure described above by diluting accordingly with distilled
water.
Stability:
When stored in a cool place (refrigerator), the stock solution of
approx. 1000 mg/l and the diluted standard solutions
(investigational concentrations) remain stable for one day.
30% H O in a calibrated or con-
®
2 2
Reagents required:
1.09122.1000 Potassium
per manganate
solution 0.02 mol/l
Titripur®
1.07209.0250 Perhydrol 30 % for
®
analysis EMSURE®
1.00716.1000 Sulfuric acid 25 %
for analysis EMSURE®
1.16754.9010 Water for analysis
EMSURE®
Chapter 5.2 | Page 14 of 23