Millipore Labscale User Manual
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Millipore Labscale User Manual

Tangential flow filtration system
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Labscale
Tangential
™
Flow Filtration System
User Guide

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Summary of Contents for Millipore Labscale

  • Page 1 Labscale Tangential ™ Flow Filtration System User Guide...
  • Page 2 Notice The information in this document is subject to change without notice and should not be construed as a commitment by Millipore Corporation. Millipore Corporation assumes no responsibility for any errors that may appear in this document. This manual is believed to be complete and accurate at the time of publication. In no event shall Millipore Corporation be liable for incidental or consequential damages in connection with or arising from the use of this manual.

  • Page 3: Table Of Contents

    Labscale Tangential Flow Filtration System User Guide Contents Introduction Operator and Equipment Safety Labscale Stirbase Setup Module set up Operating Requirements System Overview/Unpacking Lists Labscale TFF System Labscale 500 mL Reservoir Labscale Stir Base Labscale Pump Module Pellicon XL 50 Device...
  • Page 4 Labscale TFF system kit Compatible pumps and pump head Pellicon XL Devices Labscale TFF System Maintenance Labscale Stir Base Spare Parts Labscale 500 mL Reservoir Spare Parts Labscale Pump Module Spare Parts Pump Check Valve Flushing Diaphragm Pump Maintenance Electrical Schematic Labscale TFF SystemTroubleshooting...

  • Page 5: Introduction

    Labscale Tangential Flow Filtration System User Guide Introduction The Labscale TFF system is a simple, easy to use system, designed specifically for the Pellicon XL device. This modular system ® includes a 500 mL acrylic reservoir with pressure gauges, device docking manifold and retentate valve;...

  • Page 6: Labscale Stirbase Setup

    Risk of electric shocks • Unplug power cord prior to opening the blank panel from system base. • There are no user-serviceable parts inside the Labscale system. • Service should be performed only by trained and authorized personnel. Risk of rupture •...

  • Page 7: Operating Requirements

    This equipment can potentially be used with biohazardous products. Operating Requirements The system needs to be connected to electrical power and have fuses installed to operate. The Labscale system must only be uesd with the Millipore wall transfomer provided and Millipore accessories. Location Power Supply Reference...

  • Page 8: System Overview/Unpacking Lists

    System Overview/Unpacking Lists Labscale TFF System The Labscale TFF System is shipped in an overpack carton that contains three separate cartons. One carton contains the Labscale 500 mL Reservoir, one contains the Labscale Stir Base with the Labscale pump module installed, one contains the AC Adapter.

  • Page 9: Labscale 500 Ml Reservoir

    Retentate Back Pressure Valve Retentate Pressure Gauge Feed Pressure Gauge Figure 2: Labscale 500 mL Reservoir, Catalog No. XX42RES01 1 stir bar 6 feet (1.8m) of Silicone 1 shut-o 1 Millex (translucent) tubing...
  • Page 10 The following items are also packed with your Labscale 500 mL Reservoir: 6 feet (1.8 m) of Silicone (translucent) tubing 1 Shut-o Valve 1/8 in. (3 mm) ID 10 Fittings 2 x 1 ft (0.3m) of Sta-Pure® peristaltic pump tubing 1/8 in.

  • Page 11: Labscale Stir Base

    Labscale Stir Base A magnetic stirrer is installed inside the base housing. A stir bar is packed with the Labscale Stir Base unit. The speed control knob for the stirrer is located on the front of the stir base. 1 Stir Bar...

  • Page 12: Labscale Pump Module

    The Labscale Pump Module consists of a panel mounted electronic diaphragm pump and a panel mounted speed controller that can be installed in the Labscale Stir Base. The Labscale Pump Module is mounted inside the base if a complete system was purchased. If the pump unit was purchased separately, it is packed as shown in figure 5.

  • Page 13: Pellicon Xl 50 Device

    Labscale Tangential Flow Filtration System User Guide Pellicon XL 50 Device The Pellicon XL device is not included with the Labscale TFF System. It must be purchased separately. The Pellicon XL device includes an Accessories Kit that is packed in the Pellicon XL box below the device.

  • Page 14: Labscale Tff System Setup

    Labscale TFF System Setup See “Labscale 500mL Reservoir Setup” section for detailed setup instructions. RETENTATE RET IN PORT BACK PRESSURE VALVE RETENTATE TUBING DIA RECIRC COVER VENT PORT RET OUT PORT MILLEX VENT PERM 2 OUT FILTER PERMEATE TUBING...
  • Page 15 PERM 1 OUT ERVOIR T-OFF VALVE FEED PRESSURE GAUGE Figure 8: Labscale 500 mL Reservoir set up with Labscale TFF System. FEED IN PORT Figure 9: Labscale 500 mL Reservoir set up with user supplied peristaltic pump. Install the Sta-Pure (white) tubing on the pump outlet and feed in...

  • Page 16: Labscale 500 Ml Reservoir Setup

    Labscale 500 mL Reservoir Setup Note: All tubing lengths are recommended to minimize recirculation volume. Longer lengths may be used. After prolonged storage, the tubing may absorb a small volume of water. As a result, the tubing color may change from translucent to opaque, which is normal.
  • Page 17 MALE MALE L = 15 cm (6 inches) Fittings: 1 male, 1 female L = 15 cm (6 inches) Labscale Tangential Flow Filtration System User Guide Fittings: 1 male, 1 female RET IN PORT RETENTATE TUBING RET IN PORT RETENTATE TUBING...
  • Page 18 PERM 2 OUT PERMEATE TUBIN G PERM 2 OUT PERMEATE TUBIN G Figure 13: Install permeate tubing. Install Tank Outlet Valve Remove plug from the tank outlet port (TANK OUT) and insert the female luer end of the tank outlet valve over the TANK OUT port.

  • Page 19: Labscale Stir Base Set Up

    TANK OUT PORT TANK OUTLET VALVE Labscale Tangential Flow Filtration System User Guide Install Stir Bar If mixing is required, open reservoir cover and drop stir bar to the bottom of the reservoir. VENT PORT MILLEX VENT FILTER Figure 15: Install Millex vent.
  • Page 20 Figure 16: ower Connection Check Operation Remove the plugs from the pump inlet and pump outlet ports. Turn on the pump speed control, set to 2, and listen for pump motor. PUMP INLET PORT Turn off the pump speed control.

  • Page 21: Labscale Pump Module Set Up

    Labscale Tangential Flow Filtration System User Guide Labscale Pump Module Set Up Labscale Pump Module Installation If you ordered the Labscale Pump Module separately, you will need to install it in the Labscale Stir Base. WARNING: Disconnect power connection before proceeding.
  • Page 22 Figure 20: Labscale Pump Module installation Speed Control WIRE Connector Number Color + FIELD not used Black White -FIELD not used -ARM Black + ARM Pump Control Black After prolonged storage, the tubing may absorb a small volume of water.
  • Page 23 Labscale Tangential Flow Filtration System User Guide FEMALE FEMALE L = 20 cm (8 inches) (cut 2) Fittings: 4 female (2 per length) Figure 21: Set-up of pump inlet and outlet tubing, fittings and clamps. Cut one of the two Sta-Pure (white) tubing and install fittings and clamps as shown in figure 21.
  • Page 24 L = 20 cm (8 inches) (cut 2) Fittings: 4 female (2 per length) www.millipore.com TANK OUTLET VALVE PUMP INLET TUBING PUMP INLET PORT FEED IN PORT PUMP OUTLET TUBING PUMP OUTLET PORT Figure 22: Install pump inlet and outlet tubing.

  • Page 25: Install Cleaning Tubing

    Labscale Tangential Flow Filtration System User Guide Install Cleaning Tubing Install Cleaning Tubing Cut Sta-Pure (white) tubing and install fittings and clamps as shown in figure 23. FEMALE FEMALE L = 25 cm (10 inches) Fittings: 2 female Figure 23: Setup of cleaning tubing, fittings and clamps.

  • Page 26: Clean System

    DEVICE RETENTATE PORT Note: Failure to place Sta-Pure (white) tubing at the pump outlet can result in overpressurized tubing and bursting of tubing. CLEANING TUBE Clean System Disconnect retentate silicone (translucent) tubing from RET DEVICE FEED PORT IN port and place in waste collection vessel.

  • Page 27: Drain System

    Labscale Tangential Flow Filtration System User Guide Continue pumping the cleaning solution to the waste collection vessel until the level in the reservoir drops to 250 mL. Turn pump off. Reconnect the retentate silicone (translucent) tubing to the RET IN port. Turn pump on and set pump speed to 2.
  • Page 28 RETENTATE TUBING Figure 27: Setup for system draining steps 1 and 2. Replace retentate silicone (translucent) tubing in retentate port. Reconnect pump outlet Sta-Pure (white) tubing. Disconnect pump outlet Sta-Pure (white) tubing from FEED RETENTATE TUBING IN port and place in collection vessel. Open tank outlet valve, turn pump speed up to drain reservoir.

  • Page 29: Flush System

    Labscale Tangential Flow Filtration System User Guide Flush System Repeat instructions for System Cleaning substituting purified water in place of cleaning solution.

  • Page 30: Flushing Device

    Install Pellicon XL Device Remove plug from FEED, RET, PERM 1, and PERM 2 ports. Align Pellicon XL device ports with Labscale 500 mL Reservoir ports being sure that PERM and RET DEVICE ports of device and reservoir match. Press device firmLy onto reservoir ports.

  • Page 31: Flushing

    RESERVOIR PERM 2 PORT DEVICE RET PORT DEVICE PERM 2 PORT Labscale Tangential Flow Filtration System User Guide PELLICON XL DEVICE RESERVOIR PERM 1 RESERVOIR PORT RET PORT Flushing DEVICE Disconnect retentate silicone (translucent) tubing from RET PERM 1 PORT IN port and place end of retentate tubing in waste collection vessel.
  • Page 32 Reconnect the retentate silicone (translucent) tubing to the RET IN port and turn pump on - increase pump speed until feed pressure gauge reads 1.38 Bar (20 psi). Note: Check system connections for leaks—tighten connec- tions if required Adjust retentate valve restriction by slowly turning retentate...

  • Page 33: Drain System

    Labscale Tangential Flow Filtration System User Guide Drain System See “Drain System” section, page 27. Integrity Testing—Pellicon XL Device on System Integrity Test Set Up Close tank outlet valve and disconnect pump inlet tubing (silicone, translucent) from the tank outlet valve. Close the retentate valve.

  • Page 34: Preconditioning

    Add 0.1 mL of water to a 1 mL pipette. Insert bottom of pipette into the end of the permeate tubing (silicone, translucent). Measure airflow by measuring the displacement of water in the pipette for 30 seconds. Multiply the displaced volume by 2 to calculate the airflow (mL/min) and record.

  • Page 35: Concentration

    Labscale Tangential Flow Filtration System User Guide RETENTATE TUBING Figure 33: Preconditioning Setup. Drain Permeate Tubing Disconnect the permeate tubing (silicone, translucent) from the permeate outlet (PERM 2) port. Drain permeate tubing into the waste collection vessel. Reconnect the permeate tubing to the PERM 2 port.
  • Page 36 Turn pump on—increase pump speed until feed pressure gauge reads 1.38 Bar (20 psi). Note: Check system connections for leaks—tighten connec- tions if required Adjust retentate valve restriction by slowly turning retentate valve clockwise until the retentate pressure gauge reads 0.69 Bar (10 psi).

  • Page 37: Product Recovery

    Labscale Tangential Flow Filtration System User Guide Product Recovery Disconnect pump outlet tubing (Sta-Pure, white) from pump outlet port and place in product recovery collection vessel. Disconnect retentate tubing (silicone, translucent) from retentate in port. Fluid should now drain by gravity. If...

  • Page 38: Diafiltration-Vacuum

    Figure 36: Product Recovery Setup. Diafiltration—Vacuum Install Diafiltration Tubing Cut silicone (translucent) tubing and insert a male luer x 1/8. barb port in one end of the tubing as shown here. MALE L = 50 cm (20 inches) Fittings: 1 male Place the other end of the diafiltration tubing in a vessel of appropriate buffer.
  • Page 39 Labscale Tangential Flow Filtration System User Guide Connect male luer end of diafiltration tubing to the diafiltration (DIA/RECIRC) port. Plug VENT port. Insure that the end of the tubing remains below the buffer level at all times. As you remove permeate, a vacuum is created in the reservoir...

  • Page 40: Shutdown

    Shutdown Cleaning Device Recommended Cleaning Solutions Note: Follow solution manufacturer’s directions for proper handling. Please refer to the Pellicon XL Device instructions packed with the device for the most current cleaning solution information. Cleaning Concen- Temp Time Agent tration ºC...

  • Page 41: Clean System

    Labscale Tangential Flow Filtration System User Guide Clean System Disconnect retentate tubing (silicone, translucent) from RET IN port and place in waste collection vessel. Place end of permeate tubing in waste collection vessel. Open retentate valve turn counterclockwise Remove reservoir cover and fill reservoir with 500 mL of cleaning solution.
  • Page 42 PERMEATE TUBING RETENTATE TUBING Note: Check system connections for leaks—tighten connec- tions if required Adjust retentate valve restriction by slowly turning retentate valve clockwise until the retentate pressure gage reads 0.69 Bar (10 psi). Adjust pump speed and retentate valve restriction to achieve 2.07 Bar (30 psi) feed pressure and 0.69...

  • Page 43: Drain System

    Repeat flushing procedure detailed in “Flushing” section, page 31. See instructions enclosed with the Pellicon XL device for storage information. Clean Base Disconnect power. Exterior surfaces of the Labscale 500 mL Reservoir and the Labscale System Base may be cleaned with a mild soap and water solution.

  • Page 44: Specifications

    Specifications Specifications All wetted parts meet the requirements of USP Class VI testing and CFR 21 regulations. 21.6 x 27.9 cm (8.5 x 11 inches) Dimensions Height: 45.7 cm (18 inches) Weight 5.9 kg (13 lb) Maximum pump outlet pres- 5.86 Bar (85 psig)

  • Page 45: Materials Of Construction

    Labscale Tangential Flow Filtration System User Guide Materials of Construction 500 mL reservoir acrylic Reservoir cover polypropylene Reservoir cover gasket EPDM Luer fittings 316 stainless steel and polypropylene Tubing Platinum-cured silicone (translu- cent) and Sta-Pure (white) tubing Diaphragm pump housing glass-filled polypropylene...

  • Page 46: Ec Compliance Certificate

    LABSCALE XX42LSS12 XX42LSS13 Catalogue # : • Are manufactured (or supplied) by a Millipore facility whose Quality Management System is ® approved by an accredited registering body to the appropriate ISO 9001 Quality Systems Standard. • Are manufactured in accordance with applicable Millipore Standard Procedures.

  • Page 47: Labscale Tff Systemordering Information

    Description Catalog Number 115V XX42LSS11 230V XX42LSS12 230V (GB) XX42LSS13 Includes: Labscale 500 mL Reservoir, Labscale Stir Base with Labscale Pump Module installed. Labscale TFF system components are also available separately. Description Catalog Number Labscale 500 mL Reservoir XX42RES01 with gauges...

  • Page 48: Compatible Pumps And Pump Head

    XX8200230 speed, 60.600 rpm Pump head, 480 mL/min (3 roller) XX8000003 Labscale system 1/8 in. tubing kit XX80LFL25 After prolonged storage, the tubing may absorb a small volume of water. As a result, the tubing color may change from translu- cent to opaque, which is normal.

  • Page 49: Labscale Tff System Maintenance

    Labscale Tangential Flow Filtration System User Guide Labscale TFF System Maintenance Labscale Stir Base Spare Parts Item Qty per Catalog Description Number Cat # Number Fuse XX42LSP40 Thumb Screw XX42LSP41 Transformer – 115V XX42LSP42 Transfromer – 230V XX42LSP43 Transformer – 230V (Great...
  • Page 50 Item Qty per Catalog Description Number Cat. # Number Fitting, Luer-Lok XX42LSP26 Female x 10-32 St. St’l Fitting, Luer-Lok Male x XX42LSP27 10-32 St. St’l Tubing: 10-1 Platinum-cured 6 ft XX80LFL25 10-2 Sta-Pure Tubing 2 ft Plastic Fitting Kit:...

  • Page 51: Labscale Pump Module Spare Parts

    Labscale Tangential Flow Filtration System User Guide Labscale Pump Module Spare Parts Qty per Catalog Item Description Cat # Number Fitting, Male Luer-Lok x XX42LSP27 10-32 St. St’l Manifold, Pump XX42LSP80 Pump Rebuild Kit Contains: Diaphragm XX42LSP81 Check Valve Gasket, Check Valve...

  • Page 52: Pump Check Valve Flushing

    Pump Check Valve Flushing Note: The pump should be shut off during this procedure Close the tank outlet valve Disconnect the pump inlet tubing (silicone, translucent) from the tank outlet valve. Disconnect the pump outlet tubing (Sta-Pure, white) from the reservoir FEED IN port and place the end in a waste...

  • Page 53: Diaphragm Pump Maintenance

    Labscale Tangential Flow Filtration System User Guide Diaphragm Pump Maintenance Pump Panel Disassembly Disconnect the AC adapter from its power source Disconnect the AC adapter from the enclosure Remove the Pump Panel from the enclosure Disconnect the wires from the pump...
  • Page 54 2. Reinstall the pump panel on the enclosure DIAPHRAG M (1 ) G ASKETS (4 ) PUMP MAN IFO LD (1 ) PUMP SC REW S (4 ) C HEC K VALVE HO USIN G (1 ) C HEC K VALVES...

  • Page 55: Electrical Schematic

    Labscale Tangential Flow Filtration System User Guide Electrical Schematic...

  • Page 56: Labscale Tff Systemtroubleshooting

    Labscale TFF System Troubleshooting Electrical/Control Symptom Solution Pump and stirrer do Check the following: not operate AC adapter plugged into live receptacle with appropriate voltage AC adapter plugged into receptacle on the rear of system enclosure Check fuse on the rear of the system...

  • Page 57: Performance

    Performance Symptom Solution MixingStirrer speed Have a qualified technician check the too slow function of the stirrer speed controller. Flow Pump flow If the fluid moves back and forth in the too low pump inlet or pump outlet tubing, partic- ulates may be impairing the pump check valves from closing. Ensure pump check valves are clean. See check valve flushing procedure in maintenance section “Pump check Valve Flushing”...

  • Page 58: Pressure

    Pressure Symptom Solution System pressure Check the following: too low Pressure gauge calibration. Ensure tank outlet valve is open. Ensure pump inlet and outlet tubing are connected correctly. Ensure Sta-Pure (white) tubing is connected from pump outlet to feed in port (FEED IN).
  • Page 59 Labscale Tangential Flow Filtration System User Guide...

  • Page 60: Technical Assistance

    Millipore, Pellicon, Biomax and Millex are registered trademarks of Millipore Corporation. The M logo and Labscale are trademarks of Millipore Corporation. Sta-Pure is a trademark of W. L. Gore & Associates. Simriz is a trademark of Freudenberg & Company. Luer-Lok is a trademark of Becton, Dickinson and Com- pany. ISO is a trademark of The International Organization for Standardization.

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