Millipore Millicell MDCI10000 User Manual
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Millipore Millicell MDCI10000 User Manual

Digital cell imager
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User Guide
Millicell
®
Digital Cell Imager
MDCI10000
Contents
Intended Use. . . . . . . . . . . . . . . . . . . . . . . 1
Included . . . . . . . . . . . . . . . . . . . . . . . 1
Also Required (not included) . . . . . . . . . 1
Safety Information Sheet . . . . . . . . . . . . . . 2
Installation . . . . . . . . . . . . . . . . . . . . . . . . 2
Power On/Off . . . . . . . . . . . . . . . . . . . . 2
Cleaning and Maintenance. . . . . . . . . . . . . . 2
Specifications . . . . . . . . . . . . . . . . . . . . . . 2
Directions for Use . . . . . . . . . . . . . . . . . . . 3
Software Symbols . . . . . . . . . . . . . . . . 3
Getting Started . . . . . . . . . . . . . . . . . . 4
Image Analysis . . . . . . . . . . . . . . . . . . 4
Average . . . . . . . . . . . . . . . . . . . . . . . 6
Hemocytometer . . . . . . . . . . . . . . . . . . 7
Save an Image. . . . . . . . . . . . . . . . . . . 8
Quick Capture . . . . . . . . . . . . . . . . . . . 8
View Images . . . . . . . . . . . . . . . . . . . . 9
®
DCI Software. . . . . . . . 11
®
Cloud Account. . . . . . . . . . . . . . . 13
®
Troubleshooting . . . . . . . . . . . . . . . . . . . . 16
Product Ordering . . . . . . . . . . . . . . . . . . . 17
Notice. . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Contact Information . . . . . . . . . . . . . . 17
Technical Assistance . . . . . . . . . . . . . . 17
Standard Warranty . . . . . . . . . . . . . . . 17
20565601w
Rev 07/21
Network . . . . . . . . . 9

Intended Use

The Millicell
Digital Cell Imager (Millicell
®
is an intuitive imaging platform used for analysis
during routine cell culture monitoring. For research
use only.

Included

• Millicell
DCI device
®
• EdiMax Wireless USB adapter
• Millicell
DCI power cord
®
Also Required
• Ethernet cable (optional)
• Needed for Software Updates:
USB flash drive, FAT32 formatted
1 of 17
SigmaAldrich.com
®
(not included)
DCI)

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Summary of Contents for Millipore Millicell MDCI10000

  • Page 1: Table Of Contents

    SigmaAldrich.com User Guide Millicell ® Digital Cell Imager MDCI10000 Contents Intended Use..... . . 1 Included ..... . . 1 Also Required (not included) .

  • Page 2: Safety Information Sheet

    Safety Information Sheet Specifications A printed copy of the Safety Information has been Instrument Body included in the product box and is also downloadable from the Safety and Documentation section on the ABS plastic housing, Material product page at SigmaAldrich.com. This document Aluminum stage includes:...

  • Page 3: Directions For Use

    Directions for Use Software Symbols Click to Back to previous screen show/hide Settings Adjust illumination Adjust focus Coarse adjustment Fine focus Save focus settings Adjust focus to last saved position Capture Image Error or missing required field Discard Finish Home/Log out Focus Uploading WiFi...

  • Page 4: Getting Started

    Getting Started On the Home Screen choose: Work Offline Link User Account (click “+ Cloud Account”, input email address and password and press the Login button) Use an existing user account Note: When adding an account, the user has the option to setup a pin.
  • Page 5 To calculate estimate cell count click Count. The confluency and cell count masks can be adjusted. Click the arrow on the right side of the screen to open the two slide bars. Note: Once modified, the mask adjustments settings remain in place, regardless if the instrument is turned off.

  • Page 6: Average

    Average To calculate the average confluency or cell count of a series of images click on Begin Avg. The average confluency or cell count will be displayed in the top left-hand corner. Move the vessel to the desired location and click the Capture button.

  • Page 7: Hemocytometer

    Hemocytometer Click on Hemocytometer. Place a hemocytometer on the stage and position the quadrant within the field of view. Click the Capture icon. Cell count will automatically be determined. Total number of cells in the quadrant is displayed. Click Hide Outline to toggle on/off.

  • Page 8: Save An Image

    Click +/- to adjust the dilution factor to calculate cells/ml. Save an Image To save an image click Save. Images will be saved to the device (offline mode) or uploaded to the Cloud (if logged in with a cloud account and the device is connected to the Internet.

  • Page 9: View Images

    The image can be discarded or saved. See “Save an Image” on page 8. View Images On the Home Screen click View Images. Saved images can be searched using the Cell Line, Project Name, Type and/or Creator drop-down menus. Connecting to WiFi Network ®...
  • Page 10 3. Click Wireless Settings. Alternative: Connect the Millicell DCI to ® the network using an Ethernet cable (not provided). 4. Click View Networks. Note: You may need to contact your IT administrator to setup a network connection to the Millicell DCI.

  • Page 11: Upgrading Millicell Dci Software

    Upgrading Millicell DCI Software ® When a new software update is released, it can be downloaded from the product support page app.millicelldci.com. Installation could take 5-30 minutes. Do not turn the device off or power down during the installation. 1. Download the update file to your computer and drag the file onto a USB Flash drive that is FAT32 formatted.
  • Page 12 8. If successful, you will be prompted to confirm the found update file 9. If the correct file was found, click the Install button. If the incorrect file was found or an error was shown please refer to the troubleshooting section.

  • Page 13: Millicell ® Cloud Account

    Millicell Cloud Account ® Subscribing to the Millicell Cloud Account optimizes your workflow efficiency ® and enables seamless data collaboration. To subscribe and set up the account for your organization, a primary contact email address must be established. One organization account can accommodate multiple Millicell DCI devices.
  • Page 14 2. Click Sign up for an account. Add the email address and password. Read and accept the Terms of Service and Privacy Policy. Click Sign A verification code will be emailed. Enter the verification code and click Submit. Click Log in Again and sign in to complete the process.
  • Page 15 4. Click I received an access code and want to set up an organization. Note: Once your organization’s account is established, the organization owner can invite individuals to the organization account. 5. Complete the form, entering the access code from in the email. Click Create to complete the organization setup process.

  • Page 16: Troubleshooting

    Troubleshooting Problem Solution This indicates that the user account has uploads that failed to upload to the cloud. Try moving the Millicell DCI closer to the ® Caution icon appearing desired WiFi router or plug the Millicell DCI into an Ethernet ®...

  • Page 17: Product Ordering

    The applicable warranty for the products listed in this publication may be found at SigmaAldrich.com/terms. Merck, Millipore, Millicell and Sigma-Aldrich are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates. All other trademarks are the property of their respective owners. Detailed information on trademarks is available via publicly accessible resources.

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