Millipore Amicon Ultra-2 User Manual

User Guide
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Amicon Ultra-2
Centrifugal Filter Devices
for volumes up to 2 mL
For research use only;
not for use in diagnostic procedures.
Introduction
Amicon Ultra-2 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy
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concentrate recovery from dilute and complex sample matrices. The vertical design and available membrane surface area provide
fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for
50-fold concentration. Typical processing time is 10 to 60 minutes depending on Molecular Weight Cut Off (MWCO). Solute
polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter
device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is
achieved by a convenient reverse spin step after collecting the filtrate. The device can be spun in a swinging bucket or fixed angle
rotor. Amicon Ultra-2 devices are supplied non-sterile and are for single use only.
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The Amicon Ultra-2 product line includes 5 different cutoffs (Molecular Weight Cut Off, MWCO). These devices are for
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research use only and not for use in diagnostic procedures.
• Amicon Ultra 3K device — 3,000 MWCO
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• Amicon Ultra 10K device — 10,000 MWCO
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• Amicon Ultra 30K device — 30,000 MWCO
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• Amicon Ultra 50K device — 50,000 MWCO
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• Amicon Ultra 100K device — 100,000 MWCO
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Applications
• Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids (DNA/RNA samples, either
single- or double-stranded), microorganisms, column eluates, and purified samples
• Purification of macromolecular components found in tissue culture extracts and cell lysates, removal of primer, linkers, or
molecular labels from a reaction mix, and protein removal prior to HPLC
• Desalting, buffer exchange, or diafiltration
Materials Supplied
Filter Device
10K
4
00
3
00
2
00
1
00
20
Filtrate
collection
tube
Amicon Ultra-2 Centrifugal Filter Devices
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The Amicon
operation, one tube is used to collect filtrate; the other to cap
the device during concentration and subsequently to recover the
concentrated sample.
Concentrate
collection
tube
Ultra-2 device is supplied with two tubes. During
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1