Nikon Eclipse Ni-E Instruction Manual page 83

Microscopy Procedure
3
Adjust the optical path in the tube to direct 100% of the light to the binocular section.
Press the BINO optical path button on the front of the
main body.
There are three buttons on the front of the main body for
switching the optical path in the tube. The pressed button
is lit and that optical path status is set.
• BINO optical path button
• FRONT optical path
• REAR optical path button
4
Lower the condenser slightly from the uppermost position.
Turn the condenser focus knob until the condenser is
positioned at the upper limit (where it clicks to a stop),
and then lower it a little.
When used in combination with the
motorized XY stage
Be sure to perform this operation when using in
combination with the motorized XY stage. When the
condenser is left at the upper limit, the sample holder
may touch the condenser when moved.
Chapter 2
Microscopy Operations
Preparation
Phase Contrast
1 2 3 4 5 6 7
: 100% to binocular
button
: 100% to tube adapter
: 100% to rear port
Focus and Optical
Microscopy
System Adjustment
8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 ■
Binocular 100% with [BINO] button
P A _ _ 2 x _ _ _ _
B I N O _ _ _ _
[BINO] button: Binocular optical path 100%
Lowering the condenser slightly
from uppermost position
using the condenser focus knob
69
■
Z : _ _ _ _ _ 0 . 0 0 0 u m
Z M : 1 . 0 x _ F S 3 0 . 0 m m
NC B
ND
ND
11
32
8
OU T
IN