Nikon Eclipse Ni-E Instruction Manual page 62

Concurrent Use of Epi-Fluorescence/Phase Contrast Microscopy
By bringing the following optical elements into the optical
path, epi-fluorescence and phase contrast can be
simultaneously observed by microscope:
Filter cube for the epi-fluorescence cube turret
Ph objective
PH module for the condenser
• If the diascopic image is not shown under
epi-fluorescence microscopy, press the
dia-illumination ON/OFF switch to turn on the lamp.
• Remove the GIF filter if it is installed on the field
lens.
• Adjust the fluorescent image brightness with ND
filters in the epi-fluorescence attachment, and
phase contrast image brightness using ND filters in
the main body. Dia-illumination should be
sufficiently dimmed using the ND filter.
Chapter 2
Microscopy Operations
Preparation
Epi-fluorescence
Microscopy Procedure
1 2 3 4 5
48
Focusing Microscopy
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Filter cube
Ph objective
PH module
Concurrent use of epi-fluorescence/phase contrast
Brightness adjustment of fluorescent image
Adjusting brightness of phase contrast image
A
F. STO P
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microscopy
ND ND NCB
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